| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FNRA; CD49e; VLA5A; ITGA5 |
| Entrez GeneID | 3678 |
| clone | 10F6 |
| WB Predicted band size | 114kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ITGA5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于ITGA5抗体的3篇参考文献的简要总结(基于公开信息概括，非真实文献)：
1. **文献名称**：*Targeting integrin α5β1 with a novel therapeutic antibody inhibits tumor growth and metastasis in triple-negative breast cancer*
**作者**：Smith J, et al.
**摘要**：本研究开发了一种靶向ITGA5的人源化单克隆抗体，通过阻断整合素α5β1与纤维连接蛋白的相互作用，抑制三阴性乳腺癌细胞的黏附和迁移，在体内实验中显著减少肿瘤生长和肺转移。
2. **文献名称**：*ITGA5 monoclonal antibody suppresses angiogenesis by disrupting FAK/Src signaling in endothelial cells*
**作者**：Li X, et al.
**摘要**：该文献报道了一种抗ITGA5的单克隆抗体通过抑制内皮细胞中FAK/Src信号通路，显著降低血管生成能力，并在小鼠角膜模型中验证了其抗血管生成效果，为抗肿瘤治疗提供了新策略。
3. **文献名称**：*A high-affinity anti-ITGA5 antibody enhances chemotherapy sensitivity in colorectal cancer models*
**作者**：Wang Y, et al.
**摘要**：研究证明，高亲和力的ITGA5抗体可通过阻断整合素介导的细胞存活信号，增强结直肠癌细胞对5-FU化疗的敏感性，并逆转肿瘤微环境中的耐药性。
(注：以上文献为示例性内容，实际文献需通过PubMed/Google Scholar等平台以关键词“ITGA5 antibody”检索。)
The ITGA5 antibody targets integrin alpha-5 (ITGA5), a subunit of the α5β1 heterodimeric transmembrane receptor. ITGA5 pairs with integrin β1 (ITGB1) to form α5β1 integrin, a key receptor for fibronectin, mediating cell-matrix adhesion and bidirectional signaling. This integrin plays critical roles in embryonic development, angiogenesis, wound healing, and cancer progression by regulating cell migration, survival, and proliferation. Dysregulation of ITGA5 is linked to pathological processes, including tumor metastasis, fibrosis, and inflammatory diseases.
ITGA5 antibodies are widely used in research to study integrin-mediated signaling, cell adhesion dynamics, and extracellular matrix interactions. They serve as tools for detecting ITGA5 expression in tissues or cells via techniques like Western blot, immunofluorescence, and flow cytometry. Function-blocking ITGA5 antibodies can inhibit fibronectin binding, suppressing integrin-dependent cellular activities, making them valuable in exploring therapeutic strategies for cancer and fibrotic disorders.
Therapeutic applications are under investigation, as targeting ITGA5 may disrupt pathways promoting tumor invasion or abnormal angiogenesis. However, challenges remain in achieving specificity and minimizing off-target effects. Commercially available ITGA5 antibodies vary in clonality (monoclonal/polyclonal) and species reactivity, requiring validation for experimental contexts. Overall, ITGA5 antibodies are essential for elucidating integrin biology and developing targeted therapies.
×
