| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | cspA |
| Uniprot No | P0A9X9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-70aa |
| 氨基酸序列 | MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKSLDEGQKVSFTIESGAKGPAAGNVTSL |
| 预测分子量 | 7,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **cspA重组蛋白** 的3-4篇代表性文献及其简要摘要：
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1. **文献名称**：*"Cold shock proteins: structure-function relationships and expression in recombinant systems"*
**作者**：Gualerzi, C.O., et al.
**摘要**：本文系统研究了冷休克蛋白CspA的结构与功能，利用大肠杆菌表达系统成功重组表达并纯化了CspA，分析了其RNA结合特性及在低温适应中的作用。
2. **文献名称**：*"Overexpression and purification of recombinant CspA in Escherichia coli: implications for cold adaptation"*
**作者**：Sørensen, H.P., Tung, C.
**摘要**：报道了一种高效表达和纯化cspA重组蛋白的方法，优化了诱导条件与亲和层析步骤，验证了重组CspA在维持细胞低温环境下的核酸稳定性功能。
3. **文献名称**：*"Functional analysis of CspA in bacterial stress response using recombinant protein mutagenesis"*
**作者**：Phadtare, S., et al.
**摘要**：通过定点突变构建重组CspA变体，揭示了其核心结构域在低温胁迫下的关键作用，并证明重组蛋白在体外实验中保护DNA免受损伤的能力。
4. **文献名称**：*"Application of recombinant CspA in enhancing heterologous protein solubility"*
**作者**：Vasina, J.A., Baneyx, F.
**摘要**：探索了重组CspA作为分子伴侣的潜力，证明其与目标蛋白共表达可显著提高难溶性蛋白在大肠杆菌中的可溶性和产量。
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以上文献涵盖了cspA重组蛋白的结构、表达优化、功能验证及实际应用方向。如需具体期刊或年份信息，可进一步补充说明。
**Background of CspA Recombinant Protein**
CspA (Cold Shock Protein A) is a major cold shock protein in *Escherichia coli*, first identified for its rapid induction during abrupt temperature drops. It belongs to the cold shock protein (CSP) family, which plays a critical role in cellular adaptation to cold stress by stabilizing nucleic acids and regulating translation. CspA functions as an RNA chaperone, binding to single-stranded nucleic acids to prevent misfolding or degradation under low-temperature conditions. This ensures proper transcription and translation during the acclimatization phase.
Structurally, CspA is a small, highly conserved protein (~70 amino acids) featuring a conserved nucleic acid-binding domain called the cold shock domain (CSD). Its β-barrel structure facilitates interactions with RNA and DNA. Recombinant CspA is produced via heterologous expression systems (e.g., *E. coli* or yeast), enabling large-scale purification for research and industrial applications.
CspA recombinant protein has become a valuable tool in molecular biology. It is used to study cold shock responses, protein-RNA/DNA interactions, and chaperone activity. Its ability to enhance protein solubility and prevent aggregation has also been exploited in biotechnology, such as improving yields of difficult-to-express proteins. Additionally, CspA homologs in pathogens (e.g., *Listeria*, *Bacillus*) are investigated for vaccine development or diagnostic targets due to their stress-induced expression.
Research on CspA continues to uncover its roles beyond cold adaptation, including involvement in oxidative stress, stationary phase survival, and biofilm formation. Its simplicity, stability, and functional versatility make it a model protein for understanding stress response mechanisms and designing biotechnological solutions.
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