| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GDI2 |
| Uniprot No | P50395 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMNEEYDV IVLGTGLTEC ILSGIMSVNG KKVLHMDRNP YYGGESASIT PLEDLYKRFK IPGSPPESMG RGRDWNVDLI PKFLMANGQL VKMLLYTEVT RYLDFKVTEG SFVYKGGKIY KVPSTEAEAL ASSLMGLFEK RRFRKFLVYV ANFDEKDPRT FEGIDPKKTT MRDVYKKFDL GQDVIDFTGH ALALYRTDDY LDQPCYETIN RIKLYSESLA RYGKSPYLYP LYGLGELPQG FARLSAIYGG TYMLNKPIEE IIVQNGKVIG VKSEGEIARC KQLICDPSYV KDRVEKVGQV IRVICILSHP IKNTNDANSC QIIIPQNQVN RKSDIYVCMI SFAHNVAAQG KYIAIVSTTV ETKEPEKEIR PALELLEPIE QKFVSISDLL VPKDLGTESQ IFISRTYDAT THFETTCDDI KNIYKRMTGS EFDFEEMKRK KNDIYGED |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GDI2重组蛋白的3篇参考文献示例(注：文献信息为虚构模拟，仅作参考)：
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1. **文献名称**: *"Recombinant GDI2 Protein Purification and Its Role in Rho GTPase Regulation"*
**作者**: Smith J, et al.
**摘要**: 本研究报道了通过大肠杆菌表达系统高效纯化重组GDI2蛋白的方法，并验证其与Rho家族GTP酶(如RhoA和Cdc42)的相互作用，证明GDI2通过抑制GDP解离调控细胞骨架动态平衡。
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2. **文献名称**: *"Structural Insights into GDI2-Mediated Membrane Trafficking in Neurodegenerative Diseases"*
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了重组GDI2蛋白的三维结构，揭示了其C端结构域在囊泡运输中的关键作用，并探讨了GDI2突变与阿尔茨海默病中神经元突触功能障碍的潜在关联。
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3. **文献名称**: *"GDI2 Overexpression in Cancer: Recombinant Protein-Based Functional Analysis"*
**作者**: Zhang R, et al.
**摘要**: 利用重组GDI2蛋白进行体外功能实验，发现其在乳腺癌细胞中通过激活PI3K/AKT通路促进肿瘤侵袭，提示GDI2可能作为癌症治疗的潜在靶点。
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注：如需真实文献，建议通过PubMed或Web of Science检索关键词“GDI2 recombinant protein”或“GDI2 function”，并筛选近年高影响力研究。
**Background of GDI2 Recombinant Protein**
GDI2 (GDP Dissociation Inhibitor 2) is a regulatory protein belonging to the Rab GDP dissociation inhibitor (GDI) family, which plays a critical role in intracellular vesicle trafficking and membrane transport. It primarily interacts with Rab GTPases, a family of small GTP-binding proteins that control vesicle formation, motility, and fusion within eukaryotic cells. GDI2 functions as a molecular chaperone, stabilizing Rab proteins in their inactive GDP-bound state and recycling them from membranes to the cytosol, thereby maintaining Rab availability for subsequent trafficking cycles.
The recombinant form of GDI2 is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Recombinant GDI2 is widely utilized in biochemical and cell biology studies to investigate Rab-mediated processes, such as endocytosis, exocytosis, and organelle biogenesis. Its role in diseases, particularly cancer and neurodegenerative disorders, has garnered significant interest. For instance, dysregulated GDI2 expression has been linked to tumor progression, immune evasion, and synaptic vesicle dysfunction in neurological conditions.
Compared to its homolog GDI1. which is predominantly expressed in the brain, GDI2 shows broader tissue distribution and may have distinct functional roles in secretory pathways. Researchers employ recombinant GDI2 to dissect these differences, study protein-protein interactions, or screen for therapeutic agents targeting Rab-related pathologies. Its high purity and activity in *in vitro* assays make it a valuable tool for elucidating the molecular mechanisms of vesicular transport and developing targeted therapies.
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