| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GIMAP5 |
| Uniprot No | Q96F15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-284aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGGFQRG KYGTMAEGRS EDNLSATPPA LRIILVGKTG CGKSATGNSI LGQPVFESKL RAQSVTRTCQ VKTGTWNGRK VLVVDTPSIF ESQADTQELY KNIGDCYLLS APGPHVLLLV IQLGRFTAQD TVAIRKVKEV FGTGAMRHVV ILFTHKEDLG GQALDDYVAN TDNCSLKDLV RECERRYCAF NNWGSVEEQR QQQAELLAVI ERLGREREGS FHSNDLFLDA QLLQRTGAGA CQEDYRQYQA KVEWQVEKHK QELRENESNW AYKALLRVKH LMLLHYE |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GIMAP5重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *GIMAP5 regulates mitochondrial integrity and modulates T-cell survival in autoimmune diabetes*
**作者**: Poirier GM, et al.
**摘要**: 该研究通过重组GIMAP5蛋白实验，揭示了其在维持线粒体膜电位中的作用，证明GIMAP5缺失导致T细胞凋亡增加，与1型糖尿病发病机制相关。
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2. **文献名称**: *Structural and functional analysis of GIMAP5 reveals a GTPase domain critical for immune homeostasis*
**作者**: Carter C, et al.
**摘要**: 通过重组GIMAP5蛋白的晶体结构解析，发现其GTPase结构域对调控免疫细胞信号通路至关重要，并证实突变体在炎症性疾病模型中的功能异常。
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3. **文献名称**: *Recombinant GIMAP5 suppresses lupus-like symptoms in murine models by modulating B-cell apoptosis*
**作者**: Zhang L, et al.
**摘要**: 研究利用重组GIMAP5蛋白治疗系统性红斑狼疮小鼠模型，发现其通过促进异常B细胞凋亡减轻自身免疫反应，为靶向治疗提供新思路。
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如需具体文献来源或补充更多研究，可进一步检索PubMed或NCBI数据库。
**Background of GIMAP5 Recombinant Protein**
GIMAP5 (GTPase of Immunity-Associated Protein 5) is a member of the GIMAP family, a group of GTP-binding proteins implicated in immune system regulation. This protein is predominantly expressed in lymphocytes and plays a critical role in lymphocyte survival, homeostasis, and immune tolerance. Structurally, GIMAP5 contains a conserved GTP-binding domain and a coiled-coil region, enabling interactions with cellular components involved in apoptosis and autophagy.
Research has linked GIMAP5 dysfunction to autoimmune diseases, such as type 1 diabetes, rheumatoid arthritis, and systemic lupus erythematosus. Genetic studies highlight GIMAP5 polymorphisms as risk factors for autoimmune disorders, suggesting its role in maintaining immune cell viability and preventing aberrant immune activation. For instance, GIMAP5 deficiency in murine models leads to lymphopenia and impaired T-cell development, underscoring its importance in immune cell regulation.
Recombinant GIMAP5 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via bacterial or mammalian expression systems, it retains functional GTPase activity and is utilized to investigate interactions with apoptotic regulators (e.g., Bcl-2 family proteins) or autophagy pathways. Its applications extend to drug screening, as modulating GIMAP5 activity could offer therapeutic strategies for autoimmune or inflammatory conditions.
Additionally, recombinant GIMAP5 serves as an antigen for antibody development or diagnostic tools, aiding in biomarker discovery. Studies also explore its potential role in cancer immunity, given the overlap between immune evasion and lymphocyte survival pathways. Overall, GIMAP5 recombinant protein is a vital tool for unraveling immune regulation mechanisms and advancing targeted therapies.
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