| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GFRA3 |
| Uniprot No | O60609 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-374aa |
| 氨基酸序列 | DPLPTESRL MNSCLQARRK CQADPTCSAA YHHLDSCTSS ISTPLPSEEP SVPADCLEAA QQLRNSSLIG CMCHRRMKNQ VACLDIYWTV HRARSLGNYE LDVSPYEDTV TSKPWKMNLS KLNMLKPDSD LCLKFAMLCT LNDKCDRLRK AYGEACSGPH CQRHVCLRQL LTFFEKAAEP HAQGLLLCPC APNDRGCGER RRNTIAPNCA LPPVAPNCLE LRRLCFSDPL CRSRLVDFQT HCHPMDILGT CATEQSRCLR AYLGLIGTAM TPNFVSNVNT SVALSCTCRG SGNLQEECEM LEGFFSHNPC LTEAIAAKMR FHSQLFSQDW PHPTFAVMAH QNEN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GFRA3重组蛋白的3篇代表性文献示例(注：文献为假设性示例，实际需根据数据库检索结果调整)：
---
1. **标题**: *Recombinant GFRA3 protein promotes neuronal survival in vitro*
**作者**: Smith A, et al.
**摘要**: 研究通过哺乳动物细胞表达系统制备重组GFRA3蛋白，验证其与配体Artemin的结合活性，并证明其能增强背根神经节神经元在体外培养中的存活率，提示其在神经修复中的潜在应用。
2. **标题**: *Structural characterization of GFRA3 extracellular domain and its interaction with RET tyrosine kinase*
**作者**: Zhang L, et al.
**摘要**: 利用X射线晶体学解析GFRA3重组蛋白胞外区结构，揭示其与共受体RET的结合模式，为GDNF家族受体信号传导机制提供结构生物学依据。
3. **标题**: *GFRA3 overexpression in colorectal cancer: Recombinant protein-based functional analysis*
**作者**: Tanaka K, et al.
**摘要**: 通过重组GFRA3蛋白刺激结肠癌细胞系，发现其通过激活MAPK通路促进肿瘤侵袭迁移，提示GFRA3可能成为结直肠癌治疗的潜在靶点。
---
**提示**：建议通过PubMed或Web of Science以"GFRA3 recombinant"为关键词检索最新文献，重点关注其与神经发育、癌症或信号通路相关的功能研究。实际引用时需核对原文信息。
**Background of GFRA3 Recombinant Protein**
GFRA3 (GDNF Family Receptor Alpha 3) is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the GDNF receptor family, which includes four members (GFRA1-4). It functions as a co-receptor for RET tyrosine kinase, mediating signaling of the glial cell line-derived neurotrophic factor (GDNF) family ligands (GFLs), particularly artemin (ARTN). GFRA3 binds artemin with high affinity, facilitating the formation of a ligand-receptor complex that activates RET-dependent downstream pathways, such as MAPK and PI3K/AKT, which regulate cell survival, proliferation, and differentiation.
Primarily expressed in the peripheral and central nervous systems, GFRA3 plays critical roles in neural development, including neuron migration, axon guidance, and maintenance of sensory and sympathetic neurons. It is also implicated in non-neuronal tissues, such as the kidneys and reproductive organs. Dysregulation of GFRA3 signaling is associated with pathological conditions, including neuropathic pain, neurodegenerative diseases, and cancers (e.g., breast, pancreatic), where its overexpression often correlates with metastasis and poor prognosis.
Recombinant GFRA3 protein is engineered using mammalian or bacterial expression systems to study its structure, ligand interactions, and signaling mechanisms. Purified via affinity chromatography, it retains biological activity and is widely used in *in vitro* assays, drug screening, and antibody development. Researchers leverage this protein to explore therapeutic strategies targeting GFRA3-RET signaling in cancer and neurological disorders, making it a valuable tool in biomedical research and drug discovery.
×
