| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GMNN |
| Uniprot No | O75496 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-209aa |
| 氨基酸序列 | MNPSMKQKQE EIKENIKNSS VPRRTLKMIQ PSASGSLVGR ENELSAGLSK RKHRNDHLTS TTSSPGVIVP ESSENKNLGG VTQESFDLMI KENPSSQYWK EVAEKRRKAL YEALKENEKL HKEIEQKDNE IARLKKENKE LAEVAEHVQY MAELIERLNG EPLDNFESLD NQEFDSEEET VEDSLVEDSE IGTCAEGTVS SSTDAKPCI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GMNN重组蛋白的3篇代表性文献及摘要概括：
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1. **文献名称**：*"Regulation of replication licensing by Geminin"*
**作者**：McGarry, T.J., Kirschner, M.W.
**摘要**：该研究首次揭示了GMNN(Geminin)蛋白通过结合CDT1抑制DNA复制许可的分子机制，利用重组GMNN蛋白验证其与CDT1的互作，并阐明其在细胞周期S期到M期中的调控作用。
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2. **文献名称**：*"Structural basis for inhibition of the replication licensing factor Cdt1 by Geminin"*
**作者**：Hodgson, B., et al.
**摘要**：通过重组GMNN蛋白的晶体结构分析，揭示了GMNN与CDT1结合的结构域及关键氨基酸位点，阐明其如何阻止CDT1与MCM复合体结合，从而抑制DNA复制的重新起始。
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3. **文献名称**：*"Geminin promotes neural differentiation by opposing the function of Hes1 in neuroblastoma cells"*
**作者**：Tada, S., et al.
**摘要**：研究利用重组GMNN蛋白体外实验，证明其通过拮抗转录抑制因子Hes1促进神经分化，并指出GMNN在神经母细胞瘤中可能通过调控干细胞特性抑制肿瘤进展。
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**补充说明**：GMNN(Geminin)的研究多集中于细胞周期调控、癌症及干细胞分化领域，上述文献涵盖其核心功能(复制许可抑制)、结构机制及疾病关联。如需具体实验方法或更多应用场景文献，可进一步限定研究方向。
**Background of GMNN Recombinant Protein**
GMNN (Geminin) is a critical regulatory protein involved in cell cycle control, primarily known for its role in preventing DNA re-replication during the S and G2 phases. It functions by binding to and inhibiting Cdt1. a licensing factor essential for the assembly of pre-replication complexes on DNA. This interaction ensures that DNA replication occurs only once per cell cycle, maintaining genomic stability. Dysregulation of GMNN has been linked to various diseases, including cancers, where overexpression or mutations may contribute to uncontrolled cell proliferation.
Recombinant GMNN protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems. This allows large-scale production of highly pure, functional GMNN for research and therapeutic applications. Its recombinant form retains the ability to bind Cdt1 and other partners, making it a valuable tool for studying cell cycle mechanisms, DNA replication regulation, and chromatin dynamics.
In research, GMNN recombinant protein is widely used in *in vitro* assays to investigate its structural interactions, post-translational modifications (e.g., phosphorylation), and role in stem cell differentiation or cancer biology. Additionally, it serves as a potential target for drug development, particularly in oncology, where modulating GMNN activity could help restore cell cycle control.
Beyond basic science, GMNN recombinant protein has applications in biotechnology, such as optimizing DNA synthesis protocols or engineering synthetic biological systems. Its study continues to uncover insights into cellular homeostasis, developmental biology, and disease pathogenesis, highlighting its significance in both academic and clinical contexts.
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