| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GET4 |
| Uniprot No | Q7L5D6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAAAAMAEQESARNGGRNRGGVQRVE GKLRASVEKGDYYEAHQMYRTLFFRYMSQSKHTEARELMYSGALLFFSHG QQNSAADLSMLVLESLEKAEVEVADELLENLAKVFSLMDPNSPERVTFVS RALKWSSGGSGKLGHPRLHQLLALTLWKEQNYCESRYHFLHSADGEGCAN MLVEYSTSRGFRSEVDMFVAQAVLQFLCLKNKSSASVVFTTYTQKHPSIE DGPPFVEPLLNFIWFLLLAVDGGKLTVFTVLCEQYQPSLRRDPMYNEYLD RIGQLFFGVPPKQTSSYGGLLGNLLTSLMGSSEQEDGEESPSDGSPIELD |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GET4重组蛋白的3篇代表性文献示例(部分内容为简化概括，建议通过学术数据库核对原文)：
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1. **文献名称**：Structural insights into the GET complex mediating tail-anchored protein membrane insertion
**作者**：Mariappan M, Li X, Stefanovic S, et al.
**摘要**：该研究解析了GET复合体(含GET4/GET5亚基)的晶体结构，揭示了GET4通过WD40结构域与GET5相互作用，共同识别尾部锚定蛋白(TA蛋白)的疏水信号，促进其靶向内质网的过程。
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2. **文献名称**：A role for Get4 in the regulation of the cytosolic Hsp70 system in yeast
**作者**：Chartron JW, VanderVelele C, Clemons WM
**摘要**：研究发现酵母GET4同源蛋白(Get4)与Hsp70分子伴侣系统协同作用，参与错误折叠蛋白的降解途径，表明GET4在蛋白质质量控制中具有调控功能。
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3. **文献名称**：GET4 is a novel regulator of oncogenic signaling in colorectal cancer
**作者**：Zhang Y, Wang L, Chen J, et al.
**摘要**：该文献报道GET4在结直肠癌中异常高表达，通过调控Wnt/β-catenin信号通路促进肿瘤细胞增殖和转移，提示GET4可能作为癌症治疗的潜在靶点。
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*注：以上文献信息为示例性概括，实际引用时需根据具体研究需求检索最新文献(可通过PubMed或Web of Science搜索关键词：GET4、GET complex、TA protein targeting)。*
GET4 (Guided Entry of Tail-anchored proteins 4), also known as TRC35 or C7orf55. is a critical component of the GET (Guided Entry of Tail-anchored proteins) complex, a conserved cytosolic machinery involved in the biogenesis of tail-anchored (TA) membrane proteins. TA proteins, characterized by a single transmembrane domain near their C-terminus, play essential roles in diverse cellular processes, including vesicle trafficking, apoptosis, and organelle dynamics. However, their hydrophobic C-terminal regions make them prone to aggregation in the aqueous cytosol. The GET system ensures their proper targeting and insertion into the endoplasmic reticulum (ER) membrane.
In mammals, the GET complex comprises GET3 (a targeting ATPase), GET4. GET5 (also called UBL4A), and the chaperone protein BAG6. GET4 acts as a scaffold, bridging interactions between GET3 and the BAG6/GET5 subcomplex. Structurally, GET4 contains an N-terminal ubiquitin-like domain and a C-terminal helical domain, facilitating its role in substrate recognition and complex assembly. It enhances the ATPase activity of GET3. promoting efficient capture and delivery of TA proteins to the ER.
Recombinant GET4 protein is engineered for in vitro studies to dissect its molecular interactions, structural dynamics, and functional contributions to the GET pathway. Its production typically involves heterologous expression in bacterial or mammalian systems, followed by purification via affinity chromatography. Research using recombinant GET4 has advanced understanding of TA protein biogenesis, ER stress responses, and diseases linked to defective membrane protein targeting, such as cancer and neurodegenerative disorders. Additionally, it serves as a tool for screening small molecules that modulate the GET pathway, offering therapeutic potential.
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