| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | PMT3 |
| Uniprot No | P47190 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 304-602aa |
| 氨基酸序列 | LLSNSGPGDSTMPSLFQASLNGTKVGKGPRDVALGSSIISIKNQALGGALLHSHVQPFPEGSEQQQVTVYGYSDANNEWFFQRIRGVEPWTDAENKTIEFVKGGEMYRLMHRLTGKNLHTHEVPAPISKSEYEVSAYGDVDLGDYKDNWIIEIVEQVGEEDPTLLHPLSTSFRIKNSILGCYLAQSGKHLPEWGFRQGEVVCLKHASKRDKRTWWNIETHENERLPQGEDFVYPKTSFFRNFMQLNSAMMATNNALVPNPEKFDGIASSAWQWPTLNVGVRLCEWSEKSIKYFLLGSPA |
| 预测分子量 | 39.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PMT3重组蛋白的假设性参考文献示例(请注意，文献为虚拟示例，实际研究需查询权威数据库)：
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1. **文献名称**：*"Recombinant Expression and Enzymatic Characterization of PMT3 in Saccharomyces cerevisiae"*
**作者**：A. Müller et al.
**摘要**：研究利用酵母系统表达PMT3重组蛋白，分析其甲基转移酶活性，发现其对组蛋白H3的特定赖氨酸残基具有修饰功能，揭示了其在表观遗传调控中的作用。
2. **文献名称**：*"Structural and Functional Analysis of PMT3 in Cancer Cell Proliferation"*
**作者**：J. Chen et al.
**摘要**：通过大肠杆菌表达重组PMT3.结合细胞实验证明其通过甲基化信号通路蛋白促进癌细胞增殖，为靶向治疗提供依据。
3. **文献名称**：*"High-Yield Purification of Recombinant PMT3 for Drug Screening Applications"*
**作者**：R. Patel et al.
**摘要**：优化PMT3重组蛋白的纯化工艺，开发基于其酶活性的高通量抑制剂筛选平台，用于抗肿瘤药物研发。
4. **文献名称**：*"PMT3-Mediated Methylation in Bacterial Pathogenicity: Insights from Recombinant Protein Studies"*
**作者**：L. García et al.
**摘要**：探讨重组PMT3在病原菌中的表达及其对毒力因子的甲基化调控，阐明其在感染机制中的潜在作用。
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建议通过PubMed、Web of Science或Google Scholar检索实际文献，关键词可扩展为“PMT3 recombinant expression”、“PMT3 methyltransferase”或结合具体研究领域(如癌症、表观遗传学)。
**Background of PMT3 Recombinant Protein**
PMT3 (Pasteurella Multocida Toxin 3) recombinant protein is derived from the pathogenic bacterium *Pasteurella multocida*, which infects a wide range of animals and occasionally humans. The native PMT toxin family, including PMT3. is known for its role in modulating host cell signaling pathways, particularly through the activation of G protein-coupled receptors (GPCRs) and downstream effectors like Rho GTPases. PMT3 specifically disrupts cellular processes such as cytoskeletal organization, proliferation, and immune evasion, contributing to bacterial virulence.
Recombinant PMT3 is engineered using molecular cloning techniques, where the gene encoding the toxin's functional domain is expressed in heterologous systems like *E. coli* or mammalian cells. This allows large-scale production of the purified protein while eliminating its inherent toxicity. Structural studies reveal that PMT3 contains a deamidase domain critical for its enzymatic activity, enabling the modification of host proteins to subvert cellular functions.
Research on PMT3 recombinant protein focuses on unraveling its mechanism of action, particularly its role in GPCR signaling and potential applications in studying diseases linked to Rho GTPase dysregulation, such as cancer or neurological disorders. Additionally, it serves as a tool for developing inhibitors or vaccines targeting *Pasteurella*-related infections. Challenges include optimizing expression systems to maintain protein stability and activity, as well as ensuring safety in experimental settings.
Overall, PMT3 recombinant protein bridges microbiological pathogenesis and cell signaling research, offering insights into both bacterial virulence strategies and eukaryotic cellular regulation. Its study holds promise for therapeutic innovations and improved understanding of host-pathogen interactions.
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