| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GINS4 |
| Uniprot No | Q9BRT9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-223aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMTEEVDFLGQDSDGGSEEVVLTPAELIERL EQAWMNEKFAPELLESKPEIVECVMEQLEHMEENLRRAKREDLKVSIHQM EMERIRYVLSSYLRCRLMKIEKFFPHVLEKEKTRPEGEPSSLSPEELAFA REFMANTESYLKNVALKHMPPNLQKVDLFRAVPKPDLDSYVFLRVRERQE NILVEPDTDEQRDYVIDLEKGSQHLIRYKTIAPLVASGAVQLI |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GINS4重组蛋白的3篇代表性文献示例(内容为模拟概括，实际文献需根据具体研究查询)：
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1. **文献名称**：*Structural Insights into the GINS4 Subunit Function in the Eukaryotic Replication Complex*
**作者**：Smith A. et al.
**摘要**：本研究通过X射线晶体学解析了重组表达的GINS4蛋白的晶体结构，揭示了其与GINS复合体其他亚基(PSF1/2/3)的相互作用界面，并证明GINS4对维持复合体稳定性及DNA复制叉进程至关重要。
2. **文献名称**：*Recombinant GINS4 Promotes DNA Replication in vitro and Interacts with CDC45-MCM Complex*
**作者**：Tanaka K. et al.
**摘要**：作者利用大肠杆菌系统表达并纯化功能性GINS4重组蛋白，通过体外复制实验证实其与CDC45-MCM复合体的协同作用，揭示了GINS4在启动DNA复制延伸中的分子机制。
3. **文献名称**：*Dysregulation of GINS4 Expression in Cancer: Implications for Cell Proliferation*
**作者**：Zhang L. et al.
**摘要**：该研究通过重组GINS4蛋白的体外功能实验，结合癌症细胞模型，发现GINS4过表达可显著增强癌细胞增殖能力，提示其作为潜在肿瘤治疗靶点的可能性。
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如需具体文献，建议通过PubMed或Web of Science检索关键词“GINS4 recombinant protein”或“GINS4 complex structure/function”获取最新研究。
**Background of GINS4 Recombinant Protein**
The GINS complex is a critical component of eukaryotic DNA replication machinery, playing an essential role in the initiation and elongation phases of DNA synthesis. Comprising four subunits—GINS1. GINS2. GINS3. and GINS4—this evolutionarily conserved complex forms a heterotetrameric structure that stabilizes the replication fork by interacting with key factors like the minichromosome maintenance (MCM) helicase and DNA polymerase. Among these subunits, GINS4 (also termed SLD5) contributes to the structural integrity of the complex and facilitates its recruitment to replication origins.
GINS4 recombinant protein is engineered through molecular cloning and heterologous expression systems (e.g., *E. coli* or insect cells), enabling high-purity production for functional and structural studies. Its recombinant form often includes affinity tags (e.g., His-tag) for efficient purification. Researchers utilize GINS4 recombinant protein to dissect its role in replisome assembly, DNA unwinding, and replication stress responses. Structural analyses, such as X-ray crystallography or cryo-EM, rely on recombinant GINS4 to map interaction interfaces within the GINS complex and with partner proteins like Cdc45 or Mcm2-7.
Additionally, GINS4 is implicated in cell cycle regulation and cancer biology. Dysregulation of GINS subunits correlates with uncontrolled proliferation in malignancies, making GINS4 a potential biomarker or therapeutic target. Recombinant GINS4 aids in drug screening assays to identify inhibitors targeting replication vulnerabilities in cancer cells. Beyond oncology, it serves as a tool to study replication errors linked to genetic disorders.
In summary, GINS4 recombinant protein is indispensable for elucidating DNA replication mechanisms, exploring disease pathways, and advancing translational research in genomic stability and targeted therapies.
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