| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | sdrD |
| Uniprot No | O86488 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-330aa |
| 氨基酸序列 | LVGTTLIFGLGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNETTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDLQENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADIILPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLSLKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAK |
| 预测分子量 | 38.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于sdrD重组蛋白的模拟参考文献示例，供参考：
1. **文献名称**：*"Recombinant expression and functional characterization of Staphylococcus aureus SdrD protein"*
**作者**：Smith J, et al.
**摘要**：本研究成功在大肠杆菌系统中表达并纯化了重组SdrD蛋白，通过体外结合实验证实其与宿主纤维蛋白原的特异性相互作用，揭示了SdrD在金黄色葡萄球菌粘附和定植中的关键作用。
2. **文献名称**：*"Structural insights into the SdrD protein from Staphylococcus aureus: Implications for host-pathogen interactions"*
**作者**：Lee H, et al.
**摘要**：利用X射线晶体学解析了重组SdrD蛋白的晶体结构，明确了其结合域的空间构象，为靶向SdrD的抗菌药物设计提供了结构基础。
3. **文献名称**：*"Role of recombinant SdrD in vaccine development against Staphylococcus aureus infections"*
**作者**：Zhang R, et al.
**摘要**：评估重组SdrD作为疫苗抗原的潜力，小鼠模型显示其可诱导中和抗体并降低感染后的细菌载量，表明其在预防金黄色葡萄球菌感染中的应用前景。
4. **文献名称**：*"Development of a diagnostic assay using recombinant SdrD for detection of Staphylococcus aureus antibodies"*
**作者**：Brown K, et al.
**摘要**：基于重组SdrD蛋白建立了高灵敏度的ELISA检测方法，能够特异性识别临床样本中的抗SdrD抗体，为金黄色葡萄球菌感染的血清学诊断提供了新工具。
**注意**：以上内容为模拟生成，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of SdrD Recombinant Protein**
SdrD (Serine-aspartate repeat protein D) is a cell wall-anchored surface protein belonging to the microbial surface component recognizing adhesive matrix molecule (MSCRAMM) family, predominantly found in *Staphylococcus aureus*. It plays a critical role in bacterial adhesion to host extracellular matrix (ECM) proteins, such as fibrinogen, fibronectin, and desmoglein-1. facilitating colonization and infection. SdrD contains characteristic structural domains, including N-terminal ligand-binding regions, B-repeat domains involved in immune evasion, and serine-aspartate (SD) repeats that may mediate structural flexibility or interactions.
The recombinant SdrD protein is produced via genetic engineering, typically using heterologous expression systems like *E. coli* or yeast. By cloning the *sdrD* gene (excluding its transmembrane domain) into expression vectors, researchers generate soluble, purified SdrD for functional studies. Recombinant SdrD enables exploration of its molecular interactions, such as binding specificity to host ligands, and its role in *S. aureus* pathogenesis, including biofilm formation and immune modulation.
Studies on SdrD recombinant protein have highlighted its potential as a therapeutic target. Inhibiting SdrD-mediated adhesion could prevent infections, particularly in antibiotic-resistant strains. Additionally, its immunogenic properties make it a candidate for vaccine development. Structural analyses using recombinant SdrD have also advanced understanding of MSCRAMM protein mechanics, aiding the design of anti-virulence strategies. However, challenges remain in optimizing its stability and antigenicity for clinical applications. Overall, SdrD recombinant protein serves as a vital tool in unraveling staphylococcal biology and developing novel anti-infective therapies.
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