| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TAK; C-2k; CTK1; CDC2L4; PITALRE |
| Entrez GeneID | 1025 |
| clone | 1B5D10 |
| WB Predicted band size | 43kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CDK9 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3篇关于CDK9抗体的参考文献及其摘要概括：
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1. **标题**：*"CDK9 is a critical regulator of RNA polymerase II-dependent transcription in HIV-1 infection"*
**作者**：Nathanie R. Landau等
**摘要**：该研究揭示了CDK9在HIV-1转录中的核心作用，通过RNA聚合酶II磷酸化调控病毒基因表达。文中使用CDK9抗体进行免疫共沉淀(Co-IP)实验，证明其与病毒Tat蛋白的相互作用，为抗病毒治疗提供新靶点。
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2. **标题**：*"Selective inhibition of CDK9 blocks transcriptional elongation in cancer cells"*
**作者**：Peter K. Vogt等
**摘要**：研究开发了一种CDK9特异性小分子抑制剂，并利用CDK9抗体通过Western blot和免疫荧光验证其在多种癌细胞中抑制RNA聚合酶II磷酸化的效果，表明CDK9靶向治疗可阻断肿瘤生长相关基因表达。
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3. **标题**：*"Dynamic regulation of CDK9 in transcriptional control and DNA damage response"*
**作者**：Michael H. Malim等
**摘要**：本文通过染色质免疫沉淀(ChIP)结合CDK9抗体，揭示了CDK9在DNA损伤修复中的双重功能：既参与基因启动子区转录激活，也调控损伤诱导的转录停滞，提示其在基因组稳定性中的作用。
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**备注**：若需具体文献年份或期刊，建议在PubMed或Sci-Hub中搜索标题获取全文。CDK9抗体多用于机制研究(如转录调控、病毒/肿瘤治疗)，选择文献时可重点关注其应用场景(如WB、IP、ChIP)。
CDK9 (Cyclin-dependent kinase 9) is a serine/threonine kinase within the CDK family, primarily known for its role in transcriptional regulation. As a core component of the positive transcription elongation factor b (P-TEFb) complex, CDK9 phosphorylates the C-terminal domain (CTD) of RNA polymerase II (Pol II), enabling transition from transcription initiation to elongation. This activity is critical for the expression of genes involved in cell growth, differentiation, and stress responses. Dysregulation of CDK9 has been implicated in cancers, viral infections (e.g., HIV), and inflammatory diseases, making it a therapeutic target.
CDK9 antibodies are essential tools for studying its expression, localization, and interactions in cellular processes. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess CDK9 levels in disease models or drug-response studies. Some antibodies specifically recognize phosphorylated (active) forms or distinct isoforms (e.g., CDK9-42/55), aiding functional analyses. In cancer research, CDK9 antibodies help evaluate its role in oncogene expression and apoptosis resistance. Additionally, they support screening of CDK9 inhibitors, which are being explored as anticancer or antiviral agents. Validated CDK9 antibodies ensure specificity across species, often targeting conserved regions like the kinase domain. Their application advances both basic research and therapeutic development.
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