| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLTP |
| Uniprot No | Q9NZD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-209aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMALLAEHLLKPLPADKQIETGPFLEAV SHLPPFFDCLGSPVFTPIKADISGNITKIKAVYDTNPAKFRTLQNILEVE KEMYGAEWPKVGATLALMWLKRGLRFIQVFLQSICDGERDENHPNLIRVN ATKAYEMALKKYHGWIVQKIFQAALYAAPYKSDFLKALSKGQNVTEEECL EKIRLFLVNYTATIDVIYEMYTQMNAELNYKV |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLTP(糖脂转移蛋白)重组蛋白的3篇代表性文献摘要，供参考：
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1. **文献名称**：*Crystal structure of human glycolipid transfer protein (GLTP) in complex with lyso-GM3*
**作者**：D. K. Simanshu, L. Malinina, et al.
**摘要**：该研究解析了人源重组GLTP与糖脂类似物lyso-GM3复合体的晶体结构，揭示了GLTP通过疏水口袋和氢键网络选择性结合糖脂的分子机制，为理解其跨膜转运功能提供结构基础。
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2. **文献名称**：*Recombinant human GLTP: Expression, purification, and functional characterization*
**作者**：J. A. Stockert, K. B. Leslie, et al.
**摘要**：报道了通过大肠杆菌系统高效表达重组人GLTP的优化方法，经亲和层析纯化后验证其体外转移糖脂(如GM1)的活性，证实重组蛋白保留了天然构象与功能。
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3. **文献名称**：*GLTP-mediated glycosphingolipid transfer during membrane interaction*
**作者**：P. Mattjus, R. E. Brown, et al.
**摘要**：利用荧光标记的重组GLTP研究其与脂质膜的动态作用，发现pH和膜电荷影响GLTP的糖脂提取与释放效率，表明其在细胞内环境中的转运调控机制。
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*注：以上文献信息为示例，实际引用请以具体论文内容为准。如需真实文献，建议通过PubMed或Web of Science以“GLTP recombinant protein”为关键词检索。*
**Background of GLTP Recombinant Protein**
Glycolipid Transfer Protein (GLTP), a conserved lipid-binding protein, facilitates the transfer of glycolipids between cellular membranes. Initially identified in mammals, GLTP plays a regulatory role in glycolipid metabolism, membrane trafficking, and signaling. Its structure features a unique GLTP fold—a two-layered α-helical sandwich—that forms a hydrophobic pocket for glycolipid recognition. GLTP exhibits broad specificity, interacting with glycosphingolipids (e.g., cerebrosides, gangliosides) critical for cell membrane integrity and neurocellular functions.
Recombinant GLTP is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or eukaryotic expression systems. This approach ensures high purity, scalability, and customization (e.g., tagging for detection or affinity purification). Recombinant technology allows structural and functional studies, enabling researchers to dissect lipid-transfer mechanisms, membrane dynamics, and roles in diseases like lysosomal storage disorders or neurodegenerative conditions.
Research highlights GLTP's involvement in immune regulation, apoptosis, and pathogen-host interactions. For instance, GLTP modulates glycosphingolipid distribution in lipid rafts, influencing receptor signaling and viral entry. Its recombinant form is also explored as a therapeutic tool or drug target. Recent studies focus on engineering GLTP variants with enhanced stability or altered lipid specificity, aiming to optimize therapeutic potential. Overall, recombinant GLTP serves as a vital tool in membrane biology and biomedicine, bridging gaps in understanding lipid-mediated cellular processes.
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