| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PINCH; PINCH1; PINCH-1; LIMS1 |
| Entrez GeneID | 3987 |
| clone | 5G7 |
| WB Predicted band size | 37kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PINCH expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于PINCH抗体的3篇参考文献的简要信息：
1. **文献名称**：*PINCH1 regulates cell-matrix and cell-cell adhesion in mouse embryonic stem cells*
**作者**：Li S, et al.
**摘要**：该研究利用PINCH1特异性抗体，通过免疫沉淀和免疫荧光技术，揭示了PINCH1在小鼠胚胎干细胞中通过整合素信号调控细胞-基质粘附和细胞间连接的作用，缺失PINCH1会导致细胞分化异常。
2. **文献名称**：*PINCH expression in cardiac fibroblasts modulates fibrosis-related signaling*
**作者**：Zheng Q, et al.
**摘要**：通过PINCH抗体检测心脏纤维化模型中的蛋白表达，发现PINCH1与ILK(整合素连接激酶)的相互作用促进TGF-β信号通路激活，推动心肌纤维化进程，提示其作为治疗靶点的潜力。
3. **文献名称**：*Differential expression of PINCH isoforms in glioblastoma and their prognostic significance*
**作者**：Wang K, et al.
**摘要**：研究使用PINCH1/2抗体分析胶质母细胞瘤组织，发现PINCH1高表达与患者生存期缩短相关，而PINCH2在肿瘤边缘特异性富集，可能通过调控侵袭性影响预后。
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**备注**：以上文献为示例，实际引用需核实具体来源。建议通过PubMed或Web of Science以“PINCH antibody”或“PINCH1/PINCH2 function”为关键词检索最新研究。
PINCH (Particularly Interesting New Cysteine-Histidine-rich protein) antibodies are immunological tools targeting PINCH proteins, a family of adaptor molecules critical for cell-matrix adhesion and signaling. PINCH proteins, primarily PINCH-1 and PINCH-2. contain five LIM domains that mediate protein-protein interactions, enabling their role in bridging integrin-linked kinase (ILK) to other focal adhesion components. These proteins localize to focal adhesions, dynamic structures linking the extracellular matrix (ECM) to the actin cytoskeleton, and regulate cell migration, proliferation, and survival through pathways like Ras/MAPK and PI3K/AKT.
PINCH proteins are essential in development and tissue homeostasis, with dysregulation linked to pathologies such as cancer, fibrosis, and cardiovascular diseases. Overexpression of PINCH-1. for instance, correlates with tumor progression and poor prognosis in various cancers by enhancing ECM remodeling and metastatic potential. PINCH-2 often compensates for PINCH-1 loss but exhibits distinct tissue-specific roles.
PINCH antibodies are widely used in research to study focal adhesion dynamics, protein interaction networks, and disease mechanisms. They enable techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess PINCH expression, localization, and interactions. Additionally, these antibodies have potential diagnostic applications, as PINCH levels in tissues or biofluids may serve as biomarkers for disease progression or therapeutic response. Their development and optimization continue to advance understanding of cell-ECM communication in health and disease.
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