| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | acrR |
| Uniprot No | P0ACS9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-215aa |
| 氨基酸序列 | MARKTKQEAQ ETRQHILDVA LRLFSQQGVS STSLGEIAKA AGVTRGAIYW HFKDKSDLFS EIWELSESNI GELELEYQAK FPGDPLSVLR EILIHVLEST VTEERRRLLM EIIFHKCEFV GEMAVVQQAQ RNLCLESYDR IEQTLKHCIE AKMLPADLMT RRAAIIMRGY ISGLMENWLF APQSFDLKKE ARDYVAILLE MYLLCPTLRN PATNE |
| 预测分子量 | 24,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AcrR重组蛋白的3篇参考文献示例(注：以下为模拟示例，实际文献需根据具体数据库检索)：
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1. **标题**: "Structural Insights into the AcrR Transcriptional Repressor in Escherichia coli"
**作者**: Smith, J., et al.
**摘要**: 通过重组表达纯化AcrR蛋白，利用X射线晶体学解析其三维结构，揭示其与DNA结合的分子机制，阐明AcrR如何调控AcrAB-TolC外排泵基因的表达。
2. **标题**: "Functional Characterization of Recombinant AcrR in Multidrug Resistance Regulation"
**作者**: Wang, L., et al.
**摘要**: 研究重组AcrR蛋白对大肠杆菌耐药性的调控作用，通过体外凝胶迁移实验(EMSA)证实AcrR直接结合抗生素响应元件，抑制外排泵基因转录。
3. **标题**: "Overexpression of AcrR Recombinant Protein Alters Antibiotic Susceptibility in Gram-Negative Bacteria"
**作者**: Chen, X., et al.
**摘要**: 构建AcrR重组表达质粒并转化至临床耐药菌株，发现其过表达显著降低细菌对β-内酰胺类药物的耐受性，表明AcrR在耐药性反向调控中的潜在应用。
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建议通过PubMed或Web of Science以关键词“AcrR recombinant protein”或“AcrR overexpression”检索真实文献。如需具体文献协助，请提供更多研究背景或关键词。
The AcrR recombinant protein is a key regulatory component within bacterial multidrug resistance (MDR) systems, particularly in Gram-negative bacteria like *Escherichia coli*. As a member of the TetR family of transcriptional repressors, AcrR regulates the expression of the AcrAB-TolC efflux pump, a critical machinery responsible for extruding diverse antimicrobial agents, including antibiotics, detergents, and toxic compounds, out of bacterial cells. Under normal conditions, AcrR binds to the promoter region of the *acrAB-tolC* operon, suppressing its transcription. However, in the presence of specific inducing molecules (e.g., antibiotics or environmental stressors), AcrR undergoes conformational changes, dissociates from the DNA, and derepresses the operon, enabling efflux pump overexpression and enhanced drug resistance.
Recombinant AcrR proteins are engineered via cloning and expressing the *acrR* gene in heterologous systems like *E. coli* or yeast, often fused with affinity tags (e.g., His-tag) for purification. These proteins are pivotal in structural and functional studies to elucidate mechanisms of ligand binding, allosteric regulation, and DNA interaction. X-ray crystallography and biochemical assays using recombinant AcrR have revealed its dimeric structure, featuring a DNA-binding domain and a ligand-sensing domain. Researchers also employ AcrR to investigate how mutations affect antibiotic resistance phenotypes, aiding in the development of efflux pump inhibitors (EPIs) to counteract MDR. Additionally, recombinant AcrR serves as a tool in synthetic biology for designing biosensors to detect antimicrobial compounds. Its study bridges fundamental bacterial physiology and applied medical research, addressing the global challenge of antibiotic resistance.
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