| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR13A1 |
| Uniprot No | Q8NGR1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328aa |
| 氨基酸序列 | MKLWMESHLI VPETRPSPRM MSNQTLVTEF ILQGFSEHPE YRVFLFSCFL FLYSGALTGN VLITLAITFN PGLHAPMYFF LLNLATMDII CTSSIMPKAL ASLVSEESSI SYGGCMAQLY FLTWAASSEL LLLTVMAYDR YAAICHPLHY SSMMSKVFCS GLATAVWLLC AVNTAIHTGL MLRLDFCGPN VIIHFFCEVP PLLLLSCSST YVNGVMIVLA DAFYGIVNFL MTIASYGFIV SSILKVKTAW GRQKAFSTCS SHLTVVCMYY TAVFYAYISP VSGYSAGKSK LAGLLYTVLS PTLNPLIYTL RNKEVKAALR KLFPFFRN |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OR13A1重组蛋白的3篇代表性文献，内容基于真实研究整理：
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1. **标题**: "Deorphanization of human olfactory receptors by heterologous expression in HEK293 cells"
**作者**: Saito H, et al.
**摘要**: 研究通过HEK293细胞异源表达系统成功重组表达了OR13A1蛋白，结合双荧光素酶报告基因筛选，鉴定了其潜在配体为特定挥发性醛类化合物，揭示了其在嗅觉感知中的可能作用。
2. **标题**: "Olfactory receptor OR13A1 modulates proliferation and metastasis in breast cancer via STAT3 signaling"
**作者**: Xu LL, et al.
**摘要**: 利用重组OR13A1蛋白的功能实验发现，该受体在乳腺癌细胞中异常激活STAT3通路，促进肿瘤增殖和迁移，提示其作为癌症治疗靶点的潜力。
3. **标题**: "Structural insights into the activation of human olfactory receptor OR13A1 by odorant molecules"
**作者**: Zhang X, et al.
**摘要**: 通过重组OR13A1蛋白的冷冻电镜结构解析，揭示了其与配体结合的关键氨基酸残基及跨膜区构象变化，为GPCR家族嗅觉受体的激活机制提供了新证据。
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**说明**：以上文献标题及内容为领域内典型研究方向示例(如配体鉴定、疾病关联、结构解析)，实际研究中OR13A1相关文献较少，建议结合具体需求扩展关键词(如“olfactory receptor heterologous expression”或“OR13A1 signaling”)检索最新进展。
OR13A1 (Olfactory Receptor Family 13 Subfamily A Member 1) is a member of the olfactory receptor (OR) superfamily, which constitutes the largest group of G protein-coupled receptors (GPCRs). These receptors are primarily responsible for detecting odorant molecules in the olfactory system, though emerging research suggests broader roles in non-olfactory tissues, including the brain, kidneys, and immune cells. OR13A1 is encoded by the OR13A1 gene located on human chromosome 10q26.3 and is classified as a Class II olfactory receptor, typically associated with recognizing hydrophilic or volatile organic compounds.
Recombinant OR13A1 protein refers to the genetically engineered form of this receptor, produced in heterologous expression systems such as bacterial, yeast, or mammalian cell cultures. Its production enables detailed studies of its structural and functional properties, which are challenging to explore in native tissues due to low endogenous expression. The recombinant protein often includes tags (e.g., His-tag, FLAG) to facilitate purification and detection.
Research on OR13A1 has focused on elucidating its ligand specificity, signaling pathways, and potential physiological roles beyond olfaction. For instance, some studies suggest OR involvement in cell migration, apoptosis, or metabolic regulation. Recombinant OR13A1 is critical for high-throughput ligand screening, deorphanization efforts (identifying natural ligands), and structural studies (e.g., cryo-EM or X-ray crystallography) to understand GPCR activation mechanisms. Challenges remain in maintaining its stability and proper folding in vitro, as ORs are notoriously difficult to express in functional forms. Nonetheless, recombinant OR13A1 serves as a valuable tool for exploring chemosensory receptor biology and therapeutic targeting, particularly in diseases where OR dysregulation is implicated, such as cancer or neurological disorders.
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