| 纯度 | >90%SDS-PAGE. |
| 种属 | Mycobacterium |
| 靶点 | espK |
| Uniprot No | P9WJC1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-114aa |
| 氨基酸序列 | VEADEDTFYDRAQEYSQVLQRVTDVLDTCRQQKGHVFEGGLWSGGAANAANGALGANINQLMTLQDYLATVITWHRHIAGLIEQAKSDIGNNVD |
| 预测分子量 | 17.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于espK重组蛋白的3-4篇假设参考文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库检索确认)：
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1. **标题**: *Functional characterization of the espK gene in enterohemorrhagic Escherichia coli (EHEC)*
**作者**: Deng W., et al.
**摘要**: 本研究在大肠杆菌EHEC O157:H7中分析了espK基因的毒力功能，发现其编码的蛋白(重组EspK)通过调控III型分泌系统(T3SS)影响细菌对宿主细胞的粘附及效应蛋白分泌。实验表明，espK缺失突变体导致细菌致病性显著降低。
2. **标题**: *The role of EspK in Stx phage integration and virulence regulation*
**作者**: Creuzburg K., et al.
**摘要**: 文章探讨了EspK重组蛋白与Stx噬菌体整合的关联，证明EspK通过介导噬菌体DNA与EHEC基因组的整合，间接调控志贺毒素(Shiga toxin)的表达，揭示了其在细菌感染与宿主损伤中的双重作用。
3. **标题**: *Recombinant EspK induces host cell apoptosis via caspase-3 activation*
**作者**: Yang J., et al.
**摘要**: 通过纯化EspK重组蛋白并处理人结肠上皮细胞，研究发现EspK能激活caspase-3信号通路，促进细胞凋亡。该结果提示EspK可能作为EHEC逃避宿主免疫防御的关键毒力因子。
4. **标题**: *EspK as a potential diagnostic marker for EHEC infections*
**作者**: Smith R., et al.
**摘要**: 研究评估了重组EspK蛋白的免疫原性，发现感染者血清中特异性抗EspK抗体水平升高，表明其可作为EHEC感染的诊断靶标，并为疫苗开发提供新方向。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词：“espK recombinant protein”、“EHEC espK function”等。
EspK is a recombinant protein derived from bacterial pathogens, notably associated with enteric bacteria such as *Escherichia coli*. It is often linked to virulence mechanisms, particularly in pathogenic strains like enterohemorrhagic *E. coli* (EHEC) or enteropathogenic *E. coli* (EPEC). EspK is categorized as an effector protein, part of the type III secretion system (T3SS), a needle-like apparatus used by bacteria to inject virulence factors directly into host cells. These effectors manipulate host cellular processes to promote bacterial survival, colonization, or immune evasion.
The recombinant EspK protein is engineered through molecular cloning, where the *espK* gene is inserted into expression vectors (e.g., plasmids) and produced in heterologous systems like *E. coli* or yeast. This allows large-scale purification for functional studies. Researchers focus on EspK to unravel its role in pathogenesis, such as its interaction with host signaling pathways, cytoskeletal rearrangements, or modulation of inflammatory responses. Structural studies (e.g., X-ray crystallography) aim to map its functional domains, aiding in understanding how it disrupts host defenses.
EspK is also investigated for diagnostic or therapeutic applications. Antibodies against recombinant EspK may serve as biomarkers for detecting specific infections. Additionally, studying EspK-host interactions could identify targets for novel antimicrobials or vaccines. However, challenges remain in clarifying its precise mechanisms and cross-talk with other virulence factors. Overall, EspK exemplifies how recombinant proteins bridge basic microbiology and translational research, offering insights into bacterial infectivity and potential clinical tools.
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