| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DIP2A |
| Uniprot No | Q14689 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 9-127aa |
| 氨基酸序列 | EAAPLPAEVRESLAELELELSEGDITQKGYEKKRAKLLARYIPLIQGIDPSLQAENRIPGPSQTTAAAPKQQKSRPTASRDERFRSDVHTEAVQAALAKYKERKMPMPSKRRSVLVHSS |
| 预测分子量 | 42.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DIP2A重组蛋白的假设性参考文献示例(基于领域知识推测，可能需要根据实际研究调整)：
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1. **文献名称**：*DIP2A重组蛋白的体外表达及其在神经元分化中的功能研究*
**作者**：Chen et al., 2020
**摘要**：本研究通过大肠杆菌系统成功表达并纯化DIP2A重组蛋白，发现其可促进小鼠胚胎神经干细胞的突触形成，并揭示了其与Wnt信号通路的潜在相互作用。
2. **文献名称**：*DIP2A的乙酰转移酶活性及其对染色质重塑的影响*
**作者**：Garcia-Rodriguez et al., 2018
**摘要**：利用昆虫细胞表达体系获得高纯度DIP2A重组蛋白，发现其C端结构域具有乙酰转移酶活性，可能通过修饰组蛋白H3调控基因转录。
3. **文献名称**：*DIP2A重组蛋白与自闭症相关突变的相互作用分析*
**作者**：Wang et al., 2021
**摘要**：通过体外实验比较野生型与突变型DIP2A重组蛋白的功能差异，发现特定位点突变导致其与Disrupted-in-Schizophrenia 1(DISC1)蛋白的结合能力显著降低，提示其在神经发育障碍中的机制。
4. **文献名称**：*DIP2A重组蛋白的结构解析及其底物结合特性*
**作者**：Kim et al., 2019
**摘要**：采用X射线晶体学解析了DIP2A重组蛋白的N端结构域三维结构，并发现其通过疏水口袋与脂类分子结合，可能参与细胞膜相关信号传导。
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**备注**：以上内容为假设性示例，实际文献需通过学术数据库(如PubMed、Web of Science)以关键词"DIP2A recombinant protein"或"DIP2A protein purification"检索确认。若需具体文献，建议补充更详细的研究方向(如疾病模型、分子机制等)。
DIP2A (Disco-interacting protein 2 homolog A) is a conserved eukaryotic protein implicated in diverse biological processes, including neural development, chromatin regulation, and metabolic pathways. It was initially identified through its homology to the Drosophila Disco-interacting protein 2 (DIP2), which regulates neuronal growth and axonal guidance. Structurally, DIP2A contains characteristic domains such as a conserved N-terminal AMP-binding domain, a central putative acetyltransferase region, and a C-terminal region with nuclear localization signals, suggesting roles in enzymatic activity and nuclear-cytoplasmic shuttling.
Studies link DIP2A to neurodevelopmental and psychiatric disorders. Genetic variations in DIP2A are associated with autism spectrum disorder (ASD), intellectual disability, and schizophrenia, likely due to its involvement in synaptic plasticity and dendritic morphogenesis. It interacts with epigenetic regulators like CBP/p300 and modulates histone acetylation, influencing gene expression critical for neural circuit formation. Additionally, DIP2A participates in fatty acid metabolism by binding to Coenzyme A, hinting at broader roles in cellular homeostasis.
Recombinant DIP2A protein, typically produced in bacterial or mammalian expression systems, enables functional and structural studies. Its purified form facilitates investigations into enzymatic activities, protein-protein interactions, and DNA-binding properties. Researchers employ it to dissect molecular mechanisms underlying DIP2A-associated pathologies, screen therapeutic compounds, or generate antibodies for diagnostic tools. Despite progress, the precise biochemical functions of DIP2A remain partially unresolved, driving ongoing research to clarify its role in health and disease. This recombinant tool thus serves as a cornerstone for unraveling DIP2A's multifaceted biology and translational potential.
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