纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | DIP2C |
Uniprot No | Q9Y2E4 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-125aa |
氨基酸序列 | MADRSLEGMALPLEVRARLAELELELSEGDITQKGYEKKRSKLIGAYLPQPPRVDQALPQERRAPVTPSSASRYHRRRSSGSRDERYRSDVHTEAVQAALAKHKERKMAVPMPSKRRSLVVQTSM |
预测分子量 | 43.1 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DIP2C重组蛋白的3篇参考文献及其摘要概括(注:文献为模拟示例,实际引用请核实):
1. **文献名称**: "Cloning and Functional Characterization of Human DIP2C Recombinant Protein"
**作者**: Chen Y, et al.
**摘要**: 该研究成功克隆并表达了人源DIP2C重组蛋白,通过体外酶活实验发现其具有酰基转移酶活性,并证实其在神经细胞分化中的调控作用,为研究DIP2C相关发育障碍提供了分子基础。
2. **文献名称**: "Structural Insights into DIP2C via Recombinant Protein Crystallography"
**作者**: Tanaka K, et al.
**摘要**: 利用昆虫细胞系统表达并纯化DIP2C重组蛋白,通过冷冻电镜解析其三维结构,揭示了其N端酰基结合域和C端二聚化界面的关键功能,为靶向药物设计提供结构依据。
3. **文献名称**: "DIP2C Recombinant Protein Interacts with DNA Methyltransferases in Epigenetic Regulation"
**作者**: Gupta S, et al.
**摘要**: 研究通过重组DIP2C蛋白的免疫共沉淀实验,发现其与DNMT3A/3B的相互作用,表明DIP2C可能通过调控DNA甲基化参与表观遗传沉默,尤其在癌症中异常表达。
**注**:若需真实文献,建议通过PubMed或Google Scholar以关键词"DIP2C recombinant protein"检索,并筛选涉及蛋白表达、功能或结构的研究。
**Background of DIP2C Recombinant Protein**
DIP2C (Disco-interacting protein 2 homolog C) is a conserved eukaryotic protein encoded by the *DIP2C* gene, belonging to the DIP2 protein family. It shares structural homology with its homologs DIP2A and DIP2B, characterized by a conserved AMP-binding domain and potential DNA-binding motifs. Although its precise molecular mechanisms remain under investigation, DIP2C is implicated in regulating transcriptional processes, chromatin remodeling, and cellular differentiation. Studies suggest its involvement in neurodevelopment, as it interacts with proteins linked to neuronal growth and synaptic plasticity. Dysregulation of DIP2C has been associated with neurodevelopmental disorders, intellectual disabilities, and certain cancers, highlighting its biological significance.
Recombinant DIP2C protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. By cloning the *DIP2C* gene into expression vectors, researchers generate purified, bioactive protein for investigating its interactions, enzymatic activities, and role in signaling pathways. This recombinant tool has facilitated discoveries such as DIP2C’s potential involvement in modulating histone acetylation and DNA methylation, which influence gene expression patterns. Additionally, it aids in exploring disease-related mutations and screening therapeutic candidates targeting DIP2C-associated pathways.
Despite progress, gaps persist in understanding DIP2C’s full range of functions and regulatory networks. Ongoing research aims to delineate its contributions to development and disease, leveraging recombinant protein technology to advance mechanistic insights and translational applications.
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