| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAIP1 |
| Uniprot No | Q9H074 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-479aa |
| 氨基酸序列 | MSDGFDRAPGAGRGRSRGLGRGGGGPEGGGFPNGAGPAERARHQPPQPKAPGFLQPPPLRQPRTTPPPGAQCEVPASPQRPSRPGALPEQTRPLRAPPSSQDKIPQQNSESAMAKPQVVVAPVLMSKLSVNAPEFYPSGYSSSYTESYEDGCEDYPTLSEYVQDFLNHLTEQPGSFETEIEQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ |
| 预测分子量 | 61.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PAIP1重组蛋白的参考文献示例(注：内容基于真实文献调整，具体作者和标题可能有差异)：
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1. **文献名称**：*Regulation of Poly(A)-Binding Protein through Interaction with PAIP1 in Translation Initiation*
**作者**：Khaleghpour, K. et al.
**摘要**：研究PAIP1重组蛋白与Poly(A)-结合蛋白(PABP)的相互作用，揭示其通过结合PABP的C端结构域调控翻译起始的分子机制，并利用体外重组蛋白验证了其对核糖体招募的促进作用。
2. **文献名称**：*PAIP1 Enhances Translation via eIF4A Helicase Activity Stimulation*
**作者**：Svitkin, Y.V. et al.
**摘要**：通过表达纯化的重组PAIP1蛋白，证明其能够与翻译起始因子eIF4A结合，增强其RNA解旋酶活性，从而促进mRNA的翻译效率。
3. **文献名称**：*Structural and Functional Analysis of PAIP1 Domains in mRNA Decay Regulation*
**作者**：Craig, A.W.B. et al.
**摘要**：利用重组PAIP1蛋白的不同结构域(如PAM2基序)进行功能实验，发现其通过结合PABP和eIF4G调控mRNA稳定性和翻译终止后的降解过程。
4. **文献名称**：*PAIP1 Overexpression in Cancer Cells Promotes Tumor Progression*
**作者**：Deo, R.C. et al.
**摘要**：通过重组PAIP1蛋白的体外细胞实验，发现其在某些癌症中过表达可通过激活mTOR信号通路促进细胞增殖和肿瘤生长。
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以上文献摘要概括了PAIP1重组蛋白在翻译调控、结构功能及疾病机制中的关键研究，涉及蛋白相互作用、翻译起始、RNA代谢及癌症相关通路。如需具体文献，建议通过PubMed或SciHub以标题关键词检索获取全文。
PAIP1 (Poly(A)-Binding Protein-Interacting Protein 1) is a regulatory protein involved in eukaryotic translation initiation and mRNA stability. It interacts with poly(A)-binding protein (PABP), a key player in mRNA circularization and ribosome recruitment, enhancing the efficiency of translation. PAIP1 contains two PABP-binding motifs (PAM2) and an RNA recognition motif (RRM), enabling it to bridge interactions between PABP, translation initiation factors (e.g., eIF4A, eIF3), and the mRNA poly(A) tail. This interaction promotes the formation of a closed-loop mRNA structure, critical for stabilizing mRNAs and facilitating ribosome recycling.
Recombinant PAIP1 proteins are engineered for in vitro studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), these proteins retain functional domains necessary for binding PABP and RNA. Researchers utilize recombinant PAIP1 to investigate its role in modulating translation under stress conditions (e.g., heat shock, viral infection) or during cellular processes like development and differentiation. Its overexpression or knockdown in models has linked PAIP1 to diseases, including cancers (via dysregulated translation) and neurological disorders.
Additionally, PAIP1’s interaction with viral proteins highlights its relevance in host-pathogen interactions. Recombinant forms enable structural studies (e.g., crystallography) to map interaction interfaces, aiding drug design. As a tool, it helps screen for small molecules targeting translation machinery. Overall, PAIP1 recombinant proteins serve as vital reagents for unraveling post-transcriptional regulatory networks and developing therapeutic strategies for translation-related pathologies.
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