| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | lpxD |
| Uniprot No | P0CD76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MSQSTYSLEQLADFLKVEFQGNGATLLSGVEEIEEAKTAHITFLDNEKYAKHLKSSEAGAIIISRTQFQKYRDLNKNFLITSESPSLVFQKCLELFITPVDSGFPGIHPTAVIHPTAIIEDHVCIEPYAVVCQHAHVGSACHIGSGSVIGAYSTVGEHSYIHPRVVIRERVSIGKRVIIQPGAVIGSCGFGYVTSAFGQHKHLKHLGKVIIEDDVEIGANTTIDRGRFKHSVVREGSKIDNLVQIAHQVEVGQHSMIVAQAGIAGSTKIGNHVIIGGQAGITGHICIADHVIMMAQTGVTKSITSPGIYGGAPARPYQEIHRQVAKVRNLPRLEERIAALEKLVQKLEALSEQH |
| 预测分子量 | 38,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **lpxD重组蛋白** 的参考文献示例(内容为假设性概括，建议通过学术数据库验证)：
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1. **文献名称**: "Structural and Functional Analysis of LpxD, a Key Enzyme in Lipid A Biosynthesis"
**作者**: Bartling, C.M., Raetz, C.R.
**摘要**: 该研究解析了大肠杆菌LpxD的晶体结构，揭示了其作为酰基转移酶的催化机制，并通过重组蛋白实验验证了其底物特异性，为脂质A合成途径提供了关键见解。
2. **文献名称**: "Heterologous Expression and Purification of Recombinant LpxD from Pseudomonas aeruginosa"
**作者**: Zhou, Y., et al.
**摘要**: 作者在枯草芽孢杆菌中成功表达并纯化了铜绿假单胞菌的LpxD重组蛋白，优化了表达条件，并证实其酶活性，为开发针对革兰氏阴性菌的抑制剂奠定基础。
3. **文献名称**: "Role of LpxD in Bacterial Antibiotic Resistance: Insights from Recombinant Protein Studies"
**作者**: Lee, H., et al.
**摘要**: 通过重组LpxD的功能分析，发现该酶在调节脂质A修饰中的作用，过表达会导致细菌对多粘菌素的耐药性增强，揭示了其作为潜在药物靶点的重要性。
4. **文献名称**: "Immunogenicity of Recombinant LpxD Protein in Legionella pneumophila Vaccine Development"
**作者**: Cuello, F., et al.
**摘要**: 研究利用重组表达的嗜肺军团菌LpxD蛋白进行动物免疫实验，证明其可诱导保护性抗体反应，为针对军团菌的疫苗设计提供了实验依据。
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建议通过 **PubMed** 或 **Web of Science** 输入关键词(如 "lpxD recombinant protein" "expression purification")获取最新文献。如需具体文献的DOI或年份，请进一步说明!
**Background of LpxD Recombinant Protein**
LpxD is a key enzyme in the biosynthesis of lipid A, a critical component of lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria. As an acyltransferase, LpxD catalyzes the transfer of secondary acyl chains to the glucosamine backbone of lipid A during its assembly. This step is essential for maintaining bacterial membrane integrity, evading host immune responses, and mediating pathogenicity. Due to its central role in LPS synthesis, LpxD is a conserved target for developing novel antibiotics, particularly against multidrug-resistant strains like *Escherichia coli* and *Pseudomonas aeruginosa*.
Recombinant LpxD proteins are engineered through genetic cloning and heterologous expression systems (e.g., *E. coli* or yeast) to produce purified enzymes for functional and structural studies. Structural analyses reveal that LpxD adopts a characteristic left-handed β-helix fold, common in acyltransferases, with conserved catalytic residues critical for substrate binding and acyl chain transfer.
Research on LpxD focuses on elucidating its enzymatic mechanism, substrate specificity, and interactions with potential inhibitors. Small-molecule inhibitors targeting LpxD could disrupt lipid A biosynthesis, compromising bacterial viability and reducing endotoxin-induced inflammation. Additionally, recombinant LpxD serves as an antigen in vaccine development, leveraging its immunogenic properties to induce protective immunity against Gram-negative infections.
The study of LpxD recombinant proteins also aids in understanding antibiotic resistance mechanisms, as mutations in LpxD or related pathways may confer resistance to lipid A-targeting therapies. Overall, LpxD represents a promising therapeutic and biotechnological tool in combating bacterial infections and advancing antimicrobial strategies.
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