| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GOT2 |
| Uniprot No | P00505 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-430aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSSSWWTHVEMGPPDPILGVTEAFKRDTN SKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFC KASAELALGENSEVLKSGRFVTVQTISGTGALRIGASFLQRFFKFSRDVF LPKPTWGNHTPIFRDAGMQLQGYRYYDPKTCGFDFTGAVEDISKIPEQSV LLLHACAHNPTGVDPRPEQWKEIATVVKKRNLFAFFDMAYQGFASGDGDK DAWAVRHFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVES QLKILIRPMYSNPPLNGARIAAAILNTPDLRKQWLQEVKVMADRIIGMRT QLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDG RISVAGVTSSNVGYLAHAIHQVTK |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GOT2重组蛋白的3-4篇参考文献及其简要摘要：
1. **文献名称**：*Expression and purification of recombinant human mitochondrial aspartate aminotransferase (GOT2) for structural studies*
**作者**：Smith A, et al.
**摘要**：该研究报道了在大肠杆菌中高效表达并纯化人源GOT2重组蛋白的方法，通过优化诱导条件和亲和层析技术获得高纯度蛋白，并验证其酶活性和结构稳定性。
2. **文献名称**：*Functional characterization of GOT2 in cellular metabolism using a recombinant protein approach*
**作者**：Johnson R, et al.
**摘要**：利用哺乳动物细胞系统表达重组GOT2蛋白，研究其在细胞线粒体中的代谢功能，发现GOT2通过调控天冬氨酸代谢影响细胞增殖和氧化应激反应。
3. **文献名称**：*Crystal structure of recombinant GOT2 reveals insights into substrate specificity*
**作者**：Lee S, et al.
**摘要**：解析了重组GOT2蛋白的晶体结构，揭示了其底物结合口袋的关键氨基酸残基，为开发针对GOT2的代谢疾病药物提供了结构基础。
4. **文献名称**：*Role of recombinant GOT2 in cancer cell metabolism: Implications for therapeutic targeting*
**作者**：Patel K, et al.
**摘要**：通过体外实验证明，重组GOT2蛋白的过表达促进癌细胞谷氨酰胺代谢重编程，提示抑制GOT2可能成为抑制肿瘤生长的潜在策略。
(注：以上文献为示例，实际引用需根据具体论文内容调整。)
GOT2 (Glutamic-Oxaloacetic Transaminase 2), also known as aspartate aminotransferase 2 (AST2), is a mitochondrial enzyme critical for amino acid metabolism and the urea cycle. It catalyzes the reversible transfer of an amino group between aspartate and α-ketoglutarate to generate oxaloacetate and glutamate, linking carbohydrate and nitrogen metabolism. This reaction is vital for cellular energy production, nucleotide synthesis, and maintaining redox balance. GOT2 operates in the mitochondria alongside GOT1. its cytosolic isoform, forming the "malate-aspartate shuttle" to transport reducing equivalents across mitochondrial membranes, essential for ATP synthesis.
Recombinant GOT2 proteins are engineered using expression systems like *E. coli*, yeast, or mammalian cells, enabling large-scale production for research and therapeutic applications. These proteins retain the enzymatic activity and structural features of native GOT2. including homodimer assembly and pyridoxal 5'-phosphate (PLP) cofactor dependency. Their production allows detailed studies of GOT2's role in diseases such as cancer, where altered expression correlates with tumor progression and metabolic reprogramming. For instance, GOT2 deficiency in cancer cells can disrupt mitochondrial function, promoting metastasis or chemoresistance.
Additionally, recombinant GOT2 is used to investigate metabolic disorders, neurodegenerative diseases (e.g., Alzheimer’s), and liver dysfunction, where abnormal aminotransferase activity serves as a diagnostic marker. It also aids in drug discovery, screening inhibitors or modulators targeting metabolic pathways. Recent studies highlight its potential in biomarker development and personalized medicine, particularly in cancers reliant on glutamine metabolism. By providing a controllable and pure enzyme source, recombinant GOT2 advances our understanding of mitochondrial biology and therapeutic strategies for metabolic diseases.
×
