| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180 |
| Entrez GeneID | 5788 |
| clone | 2C10C11 |
| WB Predicted band size | 147.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PTPRC (AA: 928-989) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PTPRC抗体的3篇参考文献示例(文献信息为模拟概括，实际引用需核实具体来源)：
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1. **标题**: *CD45 (PTPRC) Isoforms in T Cell Signaling and Therapeutic Targeting*
**作者**: Hermiston ML et al.
**摘要**: 研究CD45不同剪接异构体在T细胞激活中的调控作用，探讨其抗体在调节免疫反应及治疗自身免疫疾病中的潜在应用。
2. **标题**: *PTPRC Antibody as a Biomarker for Leukemia Subtype Classification*
**作者**: Smith J, Brown K.
**摘要**: 通过PTPRC抗体对白血病细胞表面抗原检测，建立针对不同白血病亚型的诊断方法，验证其在临床分型中的特异性与敏感性。
3. **标题**: *Structural Insights into CD45 Phosphatase Domain via Monoclonal Antibody Binding*
**作者**: Zhang Y et al.
**摘要**: 利用单克隆抗体解析CD45蛋白酪氨酸磷酸酶结构域构象变化，揭示抗体结合对其酶活性的抑制作用，为靶向药物开发提供依据。
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注：以上内容基于PTPRC抗体的典型研究方向概括，实际文献需通过PubMed或学术数据库检索确认。
The PTPRC antibody targets the protein tyrosine phosphatase receptor type C (PTPRC), commonly known as CD45 or leukocyte common antigen (LCA). This transmembrane glycoprotein is a receptor-type tyrosine phosphatase expressed exclusively on nucleated hematopoietic cells, including T and B lymphocytes, natural killer cells, monocytes, and granulocytes. It plays a crucial role in immune cell signaling by regulating antigen receptor signaling through the dephosphorylation of Src-family kinases, thereby modulating lymphocyte activation, differentiation, and survival.
Structurally, CD45 contains a cytoplasmic phosphatase domain and an extracellular domain with variable isoforms generated by alternative splicing of exons 4-6 (A, B, and C). These isoforms (e.g., CD45RA, CD45RO) serve as markers for distinguishing naive and memory T-cell subsets. The PTPRC gene is located on chromosome 1q31-q32.
PTPRC antibodies are widely used in research and diagnostics to identify immune cell populations via flow cytometry, immunohistochemistry, or Western blot. They are essential in studying immune dysregulation, including autoimmune diseases (e.g., multiple sclerosis), leukemia/lymphoma classification (CD45 absence helps identify immature blasts), and HIV pathogenesis. Additionally, CD45 expression patterns aid in monitoring immune reconstitution post-transplant or during immunotherapy. Its conserved structure across mammals makes it a valuable cross-species marker.
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