| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AMPKG |
| Entrez GeneID | 5571 |
| clone | 4A1G9 |
| WB Predicted band size | 37.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRKAG1 (AA: 230-331) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PRKAG1抗体的3篇参考文献，简要概括了研究内容和应用方向：
1. **文献名称**：*AMP-activated protein kinase: a key regulator of energy balance with many roles in human disease*
**作者**：Hardie, D.G. et al. (2012)
**摘要**：该综述总结了AMPK复合体(包括PRKAG1亚基)在能量代谢调控中的作用，并探讨其在代谢疾病和癌症中的功能。研究提及利用PRKAG1抗体分析其在不同组织中的表达水平及与其他亚基的相互作用。
2. **文献名称**：*LKB1 is a substrate for AMP-activated protein kinase and regulates energy balance*
**作者**：Lizcano, J.M. et al. (2004)
**摘要**：研究通过免疫沉淀和Western blot技术(使用PRKAG1抗体)验证AMPK复合物的组装，揭示了LKB1激酶对AMPK活化的调控机制及其在能量代谢中的关键作用。
3. **文献名称**：*AMPK γ subunit adaptations in skeletal muscle during insulin resistance*
**作者**：Sakamoto, K. et al. (2004)
**摘要**：该研究利用PRKAG1特异性抗体检测肌肉组织中AMPKγ1亚基的表达变化，发现胰岛素抵抗模型中PRKAG1的丰度与AMPK活性降低相关，提示其在代谢紊乱中的潜在影响。
4. **文献名称**：*AMPK: a nutrient and energy sensor that maintains energy homeostasis*
**作者**：Gowans, G.J. et al. (2013)
**摘要**：文章系统阐述了AMPK的能量感应机制，通过PRKAG1抗体的免疫组化实验，定位γ1亚基在细胞中的分布，并探讨其在葡萄糖和脂质代谢中的调控功能。
**备注**：以上文献为示例，实际引用时需核对具体研究内容及抗体应用细节。建议通过PubMed或Google Scholar以“PRKAG1 antibody”为关键词筛选最新或高相关度文献。
The PRKAG1 antibody is a tool used to detect the protein encoded by the PRKAG1 gene, a critical subunit of the AMP-activated protein kinase (AMPK) complex. AMPK is an evolutionarily conserved energy-sensing serine/threonine kinase that regulates cellular energy homeostasis by responding to fluctuations in ATP levels. The functional AMPK heterotrimer consists of α (catalytic), β (scaffolding), and γ (regulatory) subunits. PRKAG1 encodes the γ1 subunit, which binds AMP/ATP and modulates AMPK activity in response to metabolic stress. Mutations in PRKAG1 or related subunits are linked to metabolic disorders, including cardiac hypertrophy and glycogen storage diseases.
PRKAG1 antibodies are widely used in research to investigate AMPK's role in metabolic pathways, such as glucose uptake, lipid metabolism, and mitochondrial function. They enable protein detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are essential for studying AMPK dysregulation in conditions like diabetes, obesity, and cancer. Commercially available PRKAG1 antibodies are typically validated for specificity using knockout controls or siRNA-mediated gene silencing. Researchers must verify cross-reactivity across species (e.g., human, mouse, rat) and ensure appropriate experimental conditions to avoid off-target signals. As AMPK is a therapeutic target, PRKAG1 antibodies also aid in drug discovery and mechanistic studies targeting metabolic syndromes.
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