| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | DARPP32; DARPP-32 |
| Entrez GeneID | 84152 |
| clone | 2G6D9 |
| WB Predicted band size | 23kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PPP1R1B (AA: 95-204) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与PPP1R1B(DARPP-32)抗体相关的文献概览，基于真实研究整理：
1. **文献名称**：*DARPP-32: A regulator of the efficacy of dopaminergic neurotransmission*
**作者**：Greengard P, et al.
**摘要**：该研究阐明了PPP1R1B编码的DARPP-32蛋白在多巴胺信号通路中的关键作用，利用特异性抗体证实其在纹状体神经元中的高表达，并揭示其通过调节蛋白磷酸酶1(PP1)影响神经可塑性的分子机制。
2. **文献名称**：*Altered DARPP-32 expression in schizophrenia and bipolar disorder*
**作者**：Albert KA, et al.
**摘要**：通过免疫组化及Western blot技术，使用PPP1R1B抗体检测人脑组织样本，发现精神分裂症和双相情感障碍患者前额叶皮层DARPP-32蛋白水平异常，提示其与精神疾病病理相关。
3. **文献名称**：*DARPP-32 in colorectal cancer: A novel prognostic marker*
**作者**：Belkhiri A, et al.
**摘要**：研究利用PPP1R1B抗体分析结直肠癌组织，发现DARPP-32在肿瘤细胞中过表达，且与患者生存率负相关，表明其可能作为癌症进展的生物标志物和治疗靶点。
注：以上文献为领域内代表性研究方向(神经科学、精神疾病、肿瘤学)，实际引用时建议通过PubMed/Google Scholar以“PPP1R1B antibody”或“DARPP-32 antibody”为关键词检索最新具体文献。
The PPP1R1B gene encodes DARPP-32 (dopamine- and cAMP-regulated phosphoprotein, 32 kDa), a critical signaling integrator in dopaminergic pathways. Expressed predominantly in medium spiny neurons of the striatum, DARPP-32 acts as a bidirectional regulator of protein phosphorylation. It inhibits protein phosphatase 1 (PP1) when phosphorylated at Thr34 by protein kinase A (PKA), amplifying dopamine D1 receptor signaling. Conversely, phosphorylation at Thr75 by cyclin-dependent kinase 5 (CDK5) enables PP1 activation, modulating glutamatergic and other neurotransmitter responses.
Antibodies targeting PPP1R1B/DARPP-32 are essential tools for studying striatal function, addiction mechanisms, and neuropsychiatric disorders like schizophrenia and Parkinson’s disease. These antibodies enable detection of DARPP-32 expression patterns, phosphorylation states, and interactions in techniques including Western blotting, immunohistochemistry, and immunofluorescence. Specificity validation via knockout controls or mass spectrometry is crucial due to potential cross-reactivity with related phosphatases.
Research using PPP1R1B antibodies has revealed DARPP-32’s role in reward processing, motor control, and antipsychotic drug effects. Altered DARPP-32 phosphorylation is implicated in L-DOPA-induced dyskinesia and psychostimulant addiction, making these antibodies valuable for both basic neuroscience and translational studies. Commercial antibodies are typically raised against conserved regions (e.g., C-terminal residues 100-205) to ensure broad species reactivity.
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