| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RB; pRb; OSRC; pp110; p105-Rb |
| Entrez GeneID | 5925 |
| clone | 2A11D4 |
| WB Predicted band size | 106.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RB1 (AA: 2106-2784) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于RB1抗体的3篇参考文献示例(内容为虚构，仅供格式参考)：
---
1. **文献名称**: "Immunohistochemical Analysis of RB1 Expression in Human Cancers"
**作者**: Smith J, et al.
**摘要**: 本研究利用RB1特异性抗体，通过免疫组化技术分析多种肿瘤组织中RB1蛋白的表达水平，发现RB1缺失与高级别肿瘤显著相关，为癌症预后评估提供了新标志物。
2. **文献名称**: "A Comparative Study of RB1 Antibodies for Western Blot and Flow Cytometry"
**作者**: Lee H, et al.
**摘要**: 系统比较了不同克隆号的RB1抗体在Western blot和流式细胞术中的性能，确定Clone XQ-23在检测磷酸化RB1时具有高特异性和灵敏度，适用于细胞周期研究。
3. **文献名称**: "Role of RB1 in Cell Cycle Arrest: Insights from Knockout Models and Antibody-Based Detection"
**作者**: Brown R, et al.
**摘要**: 通过RB1基因敲除模型结合抗体染色技术，揭示RB1蛋白在G1/S期调控中的关键作用，并验证了抗体Clone 4H7在组织切片中的可靠性。
---
(注：以上文献信息为模拟示例，实际引用请通过PubMed、Google Scholar等平台检索真实文献。)
The RB1 antibody is a crucial tool in cancer research and molecular biology, targeting the retinoblastoma protein (pRB) encoded by the RB1 tumor suppressor gene. Discovered through studies on retinoblastoma, a rare childhood eye cancer, the RB1 gene was the first identified tumor suppressor. The pRB protein regulates cell cycle progression by binding and inhibiting E2F transcription factors, thereby blocking G1-to-S phase transition. Dysregulation or mutation of RB1 disrupts cell cycle control, leading to uncontrolled proliferation and tumorigenesis, observed in retinoblastoma, osteosarcoma, and various cancers (e.g., lung, breast, bladder).
RB1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to detect pRB expression, localization, and phosphorylation status (critical for its function). They help study RB1's role in development, differentiation, and responses to therapies. Commercial RB1 antibodies are generated using recombinant RB1 proteins or synthetic peptides, validated for specificity via knockout cell lines. Researchers also employ them to explore interactions with viral oncoproteins (e.g., HPV E7) that inactivate pRB.
In diagnostics, RB1 antibodies aid in assessing RB1 pathway alterations in tumors, guiding prognostic evaluations. However, results require careful interpretation, as post-translational modifications (e.g., phosphorylation) or epitope masking may affect detection. Overall, RB1 antibodies remain indispensable for unraveling cell cycle dynamics and tumor mechanisms.
×
