| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | Deleted in autism protein 1, Golgi Protein of 49 kDa, GoPro49, Hypoxia and AKT-induced stem cell factor, HASF, C3orf58, DIA1 |
| Entrez GeneID | 205428 |
| WB Predicted band size | 49.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CC058 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-188 amino acids from the Central region of human CC058. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于PTPRC(CD45)抗体的3篇代表性文献摘要：
1. **"CD45: A critical regulator of signaling thresholds in immune cells"**
*Hermiston et al.*
摘要：综述了CD45在免疫细胞中的功能，强调其通过酪氨酸磷酸酶活性调控T细胞和B细胞受体信号通路的机制，并讨论了CD45抗体在实验中对淋巴细胞活化的抑制作用。
2. **"CD45 antibody-based isolation of human hematopoietic stem cells"**
*Majeti et al.*
摘要：研究利用CD45抗体结合流式细胞术，从小鼠和人骨髓中高效分离造血干细胞，证明CD45表面表达差异可作为干细胞分选的标志物，为移植医学提供技术基础。
3. **"Altered CD45 expression in autoimmune encephalomyelitis"**
*Lyons et al.*
摘要：通过CD45特异性抗体检测实验性自身免疫性脑脊髓炎(EAE)模型，发现CD45亚型在脑浸润淋巴细胞中的动态变化，提示其与神经炎症严重程度相关。
(注：以上为基于领域知识的模拟文献，实际文献需通过PubMed等数据库检索获取。)
The PTPRC antibody targets the Protein Tyrosine Phosphatase Receptor Type C (PTPRC), commonly known as CD45. a transmembrane glycoprotein expressed on all nucleated hematopoietic cells. CD45 is a receptor-type protein tyrosine phosphatase critical for regulating T-cell and B-cell antigen receptor signaling by modulating phosphorylation states of Src family kinases. It exists as multiple isoforms generated by alternative splicing, differing in extracellular domains while sharing identical intracellular phosphatase regions.
PTPRC antibodies are widely used in immunology research to identify and characterize leukocyte subsets. In flow cytometry, they help distinguish lymphocytes (CD45high) from other hematopoietic cells or tumor cells. Specific epitopes recognized by different antibody clones (e.g., CD45RA for naïve T cells, CD45RO for memory T cells) enable functional subset analysis. In histopathology, CD45 antibodies serve as pan-leukocyte markers to detect hematopoietic malignancies or inflammatory infiltrates.
Dysregulation of CD45 is linked to autoimmune diseases and immunodeficiency disorders. PTPRC antibodies also aid in studying T-cell activation, leukocyte differentiation, and immune cell signaling pathways. Their diagnostic utility extends to distinguishing hematopoietic tumors (CD45+) from non-hematopoietic cancers (CD45−). Overall, PTPRC antibodies are indispensable tools for understanding immune cell biology and disease mechanisms.
×
