| WB | 1/500 - 1/2000 | Human,Mouse,Rat,Rabbit |
| IF | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat,Rabbit |
| ICC | 技术咨询 | Human,Mouse,Rat,Rabbit |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat,Rabbit |
| Elisa | 1/10000 | Human,Mouse,Rat,Rabbit |
| Entrez GeneID | 929 |
| clone | 4B4F12 |
| WB Predicted band size | 40kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Immunogen | Purified recombinant fragment of human CD14 (AA: 20-214) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与CD14抗体相关的代表性文献及其摘要概括：
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1. **文献名称**：*CD14. a receptor for complexes of lipopolysaccharides (LPS) and LPS binding protein*
**作者**：Wright, S.D. et al.
**摘要**：该研究首次阐明CD14作为先天免疫系统中识别脂多糖(LPS)的关键受体，通过结合LPS结合蛋白(LBP)增强宿主对细菌感染的响应，并揭示了CD14抗体在阻断内毒素信号传导中的潜在作用。
2. **文献名称**：*Anti-CD14 antibody reduces acute graft-versus-host disease by suppressing endotoxin-mediated inflammatory responses*
**作者**：Teshima, T. et al.
**摘要**：研究证明抗CD14单克隆抗体可通过抑制LPS诱导的炎症因子(如TNF-α和IL-6)释放，显著减轻小鼠急性移植物抗宿主病(GVHD)的严重程度，提示其在免疫相关疾病中的治疗价值。
3. **文献名称**：*CD14 expression in inflammatory bowel disease: a key mediator of bacterial-induced inflammation*
**作者**：Cario, E. et al.
**摘要**：文章发现炎症性肠病(IBD)患者肠道中CD14表达显著上调，抗CD14抗体可减少肠道上皮细胞对共生菌群LPS的过度反应，为靶向CD14调控肠道炎症提供了理论依据。
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这些文献分别从分子机制、疾病治疗和临床关联角度探讨了CD14抗体的功能与应用。如需具体DOI或期刊信息，可进一步补充。
CD14 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein predominantly expressed on monocytes, macrophages, and neutrophils. It functions as a co-receptor for bacterial lipopolysaccharide (LPS) by binding to LPS-LBP (lipopolysaccharide-binding protein) complexes and transferring them to the TLR4/MD2 receptor complex, initiating innate immune responses. CD14 exists in membrane-bound (mCD14) and soluble (sCD14) forms, both critical in modulating inflammatory signaling.
CD14 antibodies are immunological tools targeting specific epitopes of the CD14 protein. Monoclonal antibodies (e.g., clones 61D3. MEM-18) are widely used in research and diagnostics to identify monocytes/macrophages via flow cytometry, immunohistochemistry, or Western blot. These antibodies help study CD14's role in LPS signaling, phagocytosis, and immune cell differentiation. Additionally, anti-CD14 antibodies have therapeutic potential; blocking CD14 can attenuate LPS-induced inflammation, making them candidates for treating sepsis or chronic inflammatory diseases.
However, CD14's dual role in pro- and anti-inflammatory pathways complicates therapeutic targeting. Soluble CD14 also serves as a biomarker in conditions like sepsis, rheumatoid arthritis, and cardiovascular diseases. Recent research explores CD14's interaction with non-bacterial ligands (e.g., DAMPs) and its influence on adaptive immunity. Despite challenges, CD14 antibodies remain vital in elucidating innate immunity mechanisms and developing precision therapies.
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