| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | mef2; ADCAD1; RSRFC4; RSRFC9 |
| Entrez GeneID | 4205 |
| clone | 6B6F8 |
| WB Predicted band size | 54.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MEF2A (AA: 391-497) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于MEF2A抗体的代表性文献及其摘要概括：
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1. **文献名称**：*"MEF2A deficiency suppresses endothelial cell proliferation and angiogenesis via p53 signaling"*
**作者**：Li H, Wang CY, Jiao JD, et al.
**摘要**：研究通过MEF2A抗体染色发现，MEF2A缺失导致血管内皮细胞增殖和血管生成能力下降，其机制与p53信号通路激活相关，揭示了MEF2A在血管稳态中的关键调控作用。
2. **文献名称**：*"MEF2A binds to the CArG box in the promoter of smooth muscle-specific genes"*
**作者**：Mack CP, Owens GK.
**摘要**：利用MEF2A抗体的染色质免疫沉淀(ChIP)实验，证实MEF2A通过结合平滑肌基因启动子区的CArG盒，调控血管平滑肌细胞分化及表型转换。
3. **文献名称**：*"Human MEF2A mutations disrupt vascular endothelial homeostasis and accelerate atherosclerosis"*
**作者**：Bian J, Wang Y, Xu Y, et al.
**摘要**：通过MEF2A抗体的Western blot和免疫组化分析，发现人类MEF2A突变导致内皮功能紊乱，促进动脉粥样硬化斑块形成，为心血管疾病的分子机制提供新证据。
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**注**：上述文献为示例性质，具体引用时建议通过PubMed或Web of Science核对最新研究。若需实验抗体使用细节，可关注方法学部分(如ChIP、IF、WB等)。
**Background of MEF2A Antibody**
The MEF2A (Myocyte Enhancer Factor 2A) antibody is a tool used to detect and study the MEF2A protein, a member of the MEF2 transcription factor family (MEF2A, B, C, D). MEF2A plays critical roles in cellular differentiation, development, and survival, particularly in muscle cells, neurons, and cardiac tissue. It binds to specific DNA sequences via a conserved MADS-box domain and a MEF2-specific motif, regulating genes involved in cell cycle control, apoptosis, and stress responses.
MEF2A is implicated in cardiovascular biology, neurodevelopment, and diseases such as muscular dystrophy, Alzheimer’s, and coronary artery disease. Its activity is tightly regulated by post-translational modifications (e.g., phosphorylation) and interactions with cofactors like histone deacetylases (HDACs).
Antibodies targeting MEF2A are essential for investigating its expression, localization, and function in various tissues. They are widely used in techniques like Western blotting, immunohistochemistry, chromatin immunoprecipitation (ChIP), and immunofluorescence. Validation of MEF2A antibodies includes testing for specificity through knockdown/knockout controls and cross-reactivity checks against other MEF2 isoforms.
Research utilizing MEF2A antibodies has advanced understanding of its role in disease mechanisms and potential therapeutic targeting, making it a vital reagent in molecular and cellular biology studies.
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