| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PDHK2; PDKII |
| Entrez GeneID | 5164 |
| clone | 3F2D7 |
| WB Predicted band size | 46.2kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDK2 (AA: 178-404) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与PDK2抗体相关的文献示例(注：文献为示例性虚构，实际引用需核实真实来源)：
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1. **文献名称**: "Characterization of a Novel Monoclonal Antibody Against PDK2 for Metabolic Studies"
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种针对PDK2蛋白的高特异性单克隆抗体，通过Western blot和免疫荧光验证其在多种细胞系中的检测能力，并发现PDK2在缺氧条件下表达显著上调，为代谢重编程研究提供工具。
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2. **文献名称**: "PDK2 Inhibition via Antibody Blockade Attenuates Tumor Growth in Colorectal Cancer Models"
**作者**: Chen L, et al.
**摘要**: 利用PDK2中和抗体抑制丙酮酸脱氢酶复合体活性，显著降低肿瘤细胞的糖酵解能力，抑制小鼠结直肠癌模型中的肿瘤增殖，提示PDK2抗体作为潜在治疗策略的可行性。
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3. **文献名称**: "Tissue-Specific Expression Profiling of PDK2 Using a Polyclonal Antibody in Diabetic Mouse Models"
**作者**: Gupta R, et al.
**摘要**: 通过兔源多克隆抗体对PDK2进行组织分布分析，发现糖尿病小鼠肝脏和骨骼肌中PDK2蛋白水平异常升高，与胰岛素抵抗相关，为代谢疾病机制研究提供依据。
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**建议**：实际文献检索可使用PubMed或Google Scholar，关键词为“PDK2 antibody + application”或结合具体研究领域(如癌症、代谢疾病)。
The pyruvate dehydrogenase kinase 2 (PDK2) antibody is a research tool designed to detect and analyze the PDK2 protein, a key regulator of cellular energy metabolism. PDK2 is one of four isoforms (PDK1-4) in the pyruvate dehydrogenase kinase family, which phosphorylates and inhibits the pyruvate dehydrogenase complex (PDC). This inhibition reduces the conversion of pyruvate to acetyl-CoA, shifting glucose metabolism from mitochondrial oxidative phosphorylation to glycolysis—a phenomenon observed in cancer (Warburg effect) and metabolic disorders. PDK2 is highly expressed in tissues with active fatty acid oxidation, such as heart and liver, and its activity is modulated by nutrient availability, hormones, and hypoxia.
PDK2 antibodies are widely used in studies exploring metabolic reprogramming in diseases like diabetes, obesity, and cancer. They enable detection of PDK2 expression via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Researchers also employ these antibodies to investigate PDK2's role in modulating insulin resistance, mitochondrial dysfunction, and tumor progression. Validated PDK2 antibodies are essential for ensuring specificity, as cross-reactivity with other PDK isoforms can confound results. By targeting PDK2. therapeutic strategies aim to restore metabolic balance, such as using PDK inhibitors to reactivate PDC and promote glucose oxidation. Overall, PDK2 antibodies are critical for advancing understanding of metabolic regulation and its implications in disease.
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