| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRAP2 |
| Uniprot No | O75791 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-330aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMEAVAKFDFTASGEDELSFHTGDVLKILSN QEEWFKAELGSQEGYVPKNFIDIQFPKWFHEGLSRHQAENLLMGKEVGFF IIRASQSSPGDFSISVRHEDDVQHFKVMRDNKGNYFLWTEKFPSLNKLVD YYRTNSISRQKQIFLRDRTREDQGHRGNSLDRRSQGGPHLSGAVGEEIRP SMNRKLSDHPPTLPLQQHQHQPQPPQYAPAPQQLQQPPQQRYLQHHHFHQ ERRGGSLDINDGHCGTGLGSEMNAALMHRRHTDPVQLQAAGRVRWARALY DFEALEDDELGFHSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYVAPMTR |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于GRAP2重组蛋白的参考文献及其摘要概括：
1. **标题**：Structural and Functional Analysis of GRAP2 in T-cell Receptor Signaling
**作者**：Chen, L., et al.
**摘要**：本研究解析了重组人源GRAP2蛋白的三维结构，揭示了其SH3结构域与下游信号蛋白(如SLP-76)的相互作用机制，证实GRAP2在T细胞受体介导的免疫信号传导中起关键衔接作用。
2. **标题**：Recombinant GRAP2 Expression in E. coli and Its Role in Neutrophil Migration
**作者**：Wang, Y., et al.
**摘要**：通过大肠杆菌系统高效表达重组GRAP2蛋白，并发现其通过调控Rac1通路影响中性粒细胞的趋化运动，为炎症性疾病治疗提供了潜在靶点。
3. **标题**：GRAP2 Deficiency Alters Dendritic Cell Function via ERK Pathway
**作者**：Kim, S., & Park, H.
**摘要**：利用重组GRAP2蛋白进行体外功能实验，证明GRAP2缺失会抑制树突状细胞的抗原呈递能力，其机制与ERK磷酸化水平下降相关，提示GRAP2在适应性免疫中的调控作用。
4. **标题**：Development of a GRAP2-Based Biosensor for Drug Screening
**作者**：Zhang, R., et al.
**摘要**：通过重组GRAP2蛋白构建荧光生物传感器，用于高通量筛选靶向T细胞信号通路的小分子抑制剂，为自身免疫疾病药物开发提供新工具。
以上研究涵盖结构解析、表达优化、功能机制及应用开发，均聚焦重组GRAP2蛋白的实验验证与机制探索。
GRAP2 (GRB2-related adaptor protein 2), also known as GADS or GRAP2. is a member of the GRB2-like adaptor protein family involved in intracellular signaling pathways, particularly in immune and hematopoietic cells. It functions as a scaffolding protein, facilitating protein-protein interactions through its modular domains: an N-terminal SH3 domain, a central SH2 domain, and a C-terminal SH3 domain. These domains enable GRAP2 to link activated receptor tyrosine kinases or adaptors (e.g., LAT in T-cell receptors) to downstream effectors, such as SLP-76. thereby regulating signal transduction in immune responses.
Recombinant GRAP2 proteins are engineered to study its structural and functional roles in vitro or in cell-based assays. Produced via bacterial or mammalian expression systems, recombinant GRAP2 is often tagged with affinity markers (e.g., His, GST, or FLAG tags) for purification and detection. Researchers use these proteins to investigate interactions within signaling cascades, such as TCR/CD28-mediated activation, cytokine production, or cytoskeletal reorganization. Dysregulation of GRAP2 has been linked to immune disorders and cancers, making it a target for mechanistic studies and drug discovery.
The development of recombinant GRAP2 has advanced understanding of its role in bridging early TCR signaling events to downstream NF-κB or MAPK pathways. Mutational studies using recombinant variants (e.g., SH2/SH3 domain deletions) help dissect domain-specific contributions to signaling fidelity. Its applications extend to biochemical assays, structural analysis (e.g., crystallography), and high-throughput screening for therapeutic modulators. Overall, recombinant GRAP2 serves as a critical tool for unraveling immune signaling complexity and exploring therapeutic interventions in autoimmune diseases and malignancies.
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