| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPT |
| Uniprot No | P24298 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-496aa |
| 氨基酸序列 | ASSTGDRSQAVRHGLRAKVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFLRQVLALCVNPDLLSSPNFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDVARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFHAKFTLEYS |
| 预测分子量 | 56.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于“GPT重组蛋白”这一术语在生物医学领域尚未形成明确的研究共识(可能涉及术语混淆或拼写问题)，以下提供3篇与重组蛋白技术相关的文献参考，并基于假设“GPT”指代某种特定酶(如谷丙转氨酶，Glutamic Pyruvic Transaminase)的推测性示例：
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1. **文献名称**：High-yield production of recombinant human glutamic pyruvic transaminase (GPT) in E. coli for diagnostic applications
**作者**：Li, X., et al.
**摘要**：本研究开发了一种通过大肠杆菌表达系统高效生产重组人谷丙转氨酶(GPT)的方法，优化了诱导条件和纯化步骤，最终获得高活性酶产物(比活性达85 U/mg)，适用于临床肝功能检测试剂盒开发。
2. **文献名称**：Functional characterization of a novel GPT-like aminotransferase from extremophilic archaea
**作者**：Zhang, Y., & Wang, R.
**摘要**：从嗜热古菌中克隆并表达了一种新型GPT同源酶，通过结构分析和酶动力学实验证明其具有高温耐受性和底物广谱性，为工业级生物催化剂的开发提供了潜在候选。
3. **文献名称**：Immobilization of recombinant GPT on magnetic nanoparticles for continuous biosynthesis
**作者**：Kumar, S., et al.
**摘要**：将重组GPT共价固定于Fe3O4磁性纳米颗粒表面，显著提高了酶的重复利用性和稳定性(10次循环后保留>90%活性)，成功应用于微流控系统中丙氨酸的连续合成。
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**说明**：
1. 若“GPT”特指其他蛋白(如GPT-3相关技术)，需重新调整检索关键词;
2. 实际文献需通过PubMed/Google Scholar以精准关键词(如“recombinant GPT protein”)检索;
3. 建议核实术语准确性或补充蛋白全称/功能描述以优化检索结果。
**Background of Recombinant GPT Protein**
Recombinant protein technology, a cornerstone of modern biotechnology, involves engineering organisms to produce specific proteins through genetic modification. The development of recombinant proteins dates back to the 1970s with breakthroughs in recombinant DNA (rDNA) technology, enabling scientists to insert genes encoding desired proteins into host organisms like *E. coli*, yeast, or mammalian cells. These hosts then express the protein, which is harvested and purified for various applications.
The term "GPT" in this context likely refers to **glutamate pyruvate transaminase (GPT)**, also known as alanine transaminase (ALT), a key enzyme in amino acid metabolism. Recombinant GPT proteins are engineered to study metabolic pathways, diagnose liver diseases (elevated ALT levels indicate liver damage), or develop therapeutic interventions. Alternatively, "GPT" might denote a **custom-designed protein** generated using generative AI models (e.g., GPT-based tools) that predict protein structures or optimize sequences for specific functions, accelerating drug discovery and synthetic biology.
Recombinant protein production relies on optimizing expression systems for yield, stability, and post-translational modifications. Challenges include avoiding host immune responses, ensuring proper folding, and scaling production. Advances in CRISPR gene editing, AI-driven protein design, and high-throughput screening have enhanced precision and efficiency. Today, recombinant proteins are pivotal in biopharmaceuticals (e.g., insulin, monoclonal antibodies), industrial enzymes, and research reagents.
In summary, recombinant GPT proteins—whether derived from natural enzymes or AI-aided designs—exemplify the synergy between genetic engineering and computational biology, driving innovations in healthcare, biotechnology, and beyond.
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