| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPNMB |
| Uniprot No | Q14956 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-474aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSAKRFHDVLGNERPSAYMREHNQLNGWS SDENDWNEKLYPVWKRGDMRWKNSWKGGRVQAVLTSDSPALVGSNITFAV NLIFPRCQKEDANGNIVYEKNCRNEAGLSADPYVYNWTAWSEDSDGENGT GQSHHNVFPDGKPFPHHPGWRRWNFIYVFHTLGQYFQKLGRCSVRVSVNT ANVTLGPQLMEVTVYRRHGRAYVPIAQVKDVYVVTDQIPVFVTMFQKNDR NSSDETFLKDLPIMFDVLIHDPSHFLNYSTINYKWSFGDNTGLFVSTNHT VNHTYVLNGTFSLNLTVKAAAPGPCPPPPPPPRPSKPTPSLGPAGDNPLE LSRIPDENCQINRYGHFQATITIVEGILEVNIIQMTDVLMPVPWPESSLI DFVVTCQGSIPTEVCTIISDPTCEITQNTVCSPVDVDEMCLLTVRRTFNG SGTYCVNLTLGDDTSLALTSTLISVP |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Glycoprotein nonmetastatic melanoma protein B (GPNMB) promotes the progression of hepatocellular carcinoma by regulating the NF-κB pathway"**
- *作者：Li Y, et al.*
- 摘要：该研究探讨了重组GPNMB蛋白在肝癌细胞中的促癌机制，发现其通过激活NF-κB通路增强肿瘤细胞增殖和转移能力。
2. **"Recombinant GPNMB accelerates skin wound healing by promoting fibroblast migration and angiogenesis"**
- *作者：Wang X, et al.*
- 摘要：研究通过动物模型验证重组GPNMB蛋白在皮肤修复中的作用，表明其通过刺激成纤维细胞迁移和血管生成缩短伤口愈合时间。
3. **"GPNMB as a neuroprotective factor in Alzheimer's disease: Recombinant protein mitigates amyloid-β toxicity in vitro"**
- *作者：Suzuki M, et al.*
- 摘要：体外实验显示重组GPNMB蛋白可减少β淀粉样蛋白对神经元的毒性，提示其在阿尔茨海默病治疗中的潜在应用。
4. **"Production and characterization of functional recombinant GPNMB for therapeutic targeting in cancer"**
- *作者：Abdou AG, et al.*
- 摘要：研究报道了重组GPNMB蛋白的大肠杆菌表达系统及纯化方法，并验证其与抗体药物偶联物(ADC)结合后对肿瘤细胞的靶向杀伤效果。
Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB) is a transmembrane glycoprotein implicated in diverse physiological and pathological processes. It belongs to the NMB/pMEL family and is encoded by the *GPNMB* gene, initially identified for its overexpression in nonmetastatic melanoma cells. Structurally, GPNMB contains an N-terminal signal peptide, an integrin-binding RGD motif, a polycystic kidney disease (PKD) domain, a transmembrane region, and a cytoplasmic tail. These domains enable its roles in cell adhesion, migration, differentiation, and immune modulation.
Recombinant GPNMB protein is produced via genetic engineering in host systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research. Its RGD motif facilitates interactions with integrins, influencing cell-matrix communication, while the PKD domain may mediate homophilic cell interactions. Dysregulation of GPNMB is linked to cancer progression, metastasis, and immunosuppression by promoting tumor cell survival, angiogenesis, and immune evasion. It is also associated with neurodegenerative diseases, notably Parkinson’s, where it enhances autophagy and clearance of toxic protein aggregates like α-synuclein.
In therapeutic contexts, GPNMB is a target for antibody-drug conjugates (e.g., glembatumumab vedotin) in clinical trials for cancers. As a biomarker, its elevated expression in tumors or bodily fluids correlates with disease severity and prognosis. Additionally, recombinant GPNMB is studied for tissue repair applications due to its role in osteoblast differentiation and neuroprotection. Research continues to unravel its dual roles in tissue homeostasis and disease, highlighting its potential as a multifunctional therapeutic agent or diagnostic tool.
×
