| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPC4 |
| Uniprot No | O75487 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 401-529aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSSLP SNVCNDERMA AGNGNEDDCW NGKGKSRYLF AVTGNGLANQ GNNPEVQVDT SKPDILILRQ IMALRVMTSK MKNAYNGNDV DFFDISDESS GEGSGSGCEY QQCPSEFDYN ATDHAGKSAN EKADS |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于 **GPC4重组蛋白** 的模拟参考文献示例(注：部分文献为虚构概括，实际研究需查阅具体数据库)：
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1. **文献名称**: *"Recombinant GPC4 Protein Enhances Wnt/β-catenin Signaling in Stem Cell Differentiation"*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过大肠杆菌表达系统制备重组GPC4蛋白，证明其能够结合Wnt配体并增强β-catenin信号通路活性，促进胚胎干细胞的神经分化，为再生医学提供潜在工具。
2. **文献名称**: *"Structural Characterization of Glypican-4 (GPC4) and Its Role in Neuronal Synapse Formation"*
**作者**: Smith J, et al.
**摘要**: 利用X射线晶体学解析了重组人源GPC4的胞外结构域，发现其通过HS链(硫酸乙酰肝素)介导突触前蛋白聚集，揭示了GPC4在神经元突触发育中的关键作用。
3. **文献名称**: *"GPC4 as a Serum Biomarker: Recombinant Protein-Based ELISA for Early Cancer Detection"*
**作者**: Brown K, et al.
**摘要**: 开发了基于重组GPC4蛋白的ELISA检测方法，发现其在胰腺癌患者血清中显著高表达，提示GPC4可能作为新型液体活检标志物。
4. **文献名称**: *"Targeting GPC4 with Recombinant Antibody-Drug Conjugates Suppresses Tumor Growth"*
**作者**: Lee H, et al.
**摘要**: 构建靶向GPC4的重组抗体-药物偶联物(ADC)，在结直肠癌小鼠模型中显著抑制肿瘤增殖，表明GPC4是癌症治疗的潜在靶点。
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**建议**：实际研究中，可通过 **PubMed** 或 **Google Scholar** 搜索关键词“recombinant GPC4 protein”、“glypican-4 expression”等获取最新文献。部分真实相关研究可能涉及GPC4在癌症、干细胞或病毒结合(如SARS-CoV-2相关研究)中的功能。
**Background of GPC4 Recombinant Protein**
Glypican-4 (GPC4) is a member of the glypican family, a group of heparan sulfate proteoglycans (HSPGs) anchored to the cell membrane via glycosylphosphatidylinositol (GPI). These proteins play critical roles in modulating cellular signaling by interacting with growth factors, receptors, and extracellular matrix components. GPC4 is particularly notable for its involvement in developmental processes, tissue homeostasis, and disease pathways, including cancer and neurological disorders.
Recombinant GPC4 protein is engineered using biotechnological methods, typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications, such as heparan sulfate chain addition. This engineered protein retains functional domains essential for ligand binding, enabling researchers to study its interactions with signaling molecules like Wnts, FGFs, and Hedgehog proteins. Its soluble form is often utilized in structural studies, ligand-receptor assays, and therapeutic development.
In cancer biology, GPC4 has dual roles: it can act as a tumor suppressor or promoter depending on context. For example, it regulates Wnt/β-catenin signaling, a pathway frequently dysregulated in cancers. In neurodegenerative diseases, GPC4 interacts with amyloid-beta peptides, influencing their aggregation in Alzheimer’s disease. Additionally, GPC4 is implicated in metabolic disorders and fibrosis, highlighting its broad biomedical relevance.
The availability of recombinant GPC4 has advanced drug discovery, enabling high-throughput screening for inhibitors or enhancers of its activity. It also serves as a tool for antibody production and diagnostic assay development. Ongoing research aims to clarify its mechanistic roles and explore its potential as a therapeutic target or biomarker, underscoring its significance in both basic and applied biomedical sciences.
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