| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPI |
| Uniprot No | P06744 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-558aa |
| 氨基酸序列 | MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPI重组蛋白的3篇参考文献示例(注：内容为模拟虚构，仅供参考)：
1. **文献名称**：*"Efficient Production of Recombinant GPI-Anchored Proteins in Mammalian Cell Systems"*
**作者**：Lee, S. et al.
**摘要**：研究提出了一种基于HEK293细胞的高效表达系统，用于生产功能性GPI锚定重组蛋白，并验证其在细胞膜定位及药物靶向递送中的应用潜力。
2. **文献名称**：*"Structural and Functional Analysis of Engineered GPI-Anchored Antigens for Vaccine Development"*
**作者**：Martinez, R. & Kumar, P.
**摘要**：通过基因工程技术将病毒抗原与GPI锚定结构域融合，成功在CHO细胞中表达重组蛋白，证明其可自发整合到脂质体膜并增强免疫原性。
3. **文献名称**：*"A Novel Method for Purification of GPI-Linked Recombinant Proteins Using Phospholipase Cleavage"*
**作者**：Chen, H. et al.
**摘要**：开发了一种基于磷脂酶D的纯化策略，可从哺乳动物细胞培养液中高效分离具有完整GPI结构的重组蛋白，为大规模制备提供新方法。
如需真实文献，建议检索PubMed或Web of Science，关键词：**"recombinant GPI-anchored protein"** / **"GPI-anchored protein expression"**。
Glycosylphosphatidylinositol (GPI)-anchored proteins are a class of membrane-associated proteins tethered to the cell surface via a glycolipid anchor. The GPI anchor, synthesized in the endoplasmic reticulum, consists of a phosphatidylinositol lipid linked to a conserved glycan core and a terminal phosphoethanolamine that covalently attaches to the protein’s C-terminus. This post-translational modification occurs in eukaryotes, including mammals, yeast, and parasites, and serves as an alternative to transmembrane domains for anchoring proteins to membranes. GPI-anchored proteins play critical roles in cell signaling, adhesion, immune response, and membrane trafficking. Their unique localization in lipid rafts—dynamic membrane microdomains enriched in cholesterol and sphingolipids—facilitates spatial organization and functional clustering for cellular communication.
Recombinant GPI-anchored proteins are engineered using biotechnological approaches to mimic native structures. These proteins are produced by expressing modified genes in host systems (e.g., mammalian, insect, or yeast cells) capable of post-translational GPI anchoring. Applications span biomedical research, including vaccine development (e.g., malaria antigens), drug discovery (targeting GPI-related pathways in cancers or infectious diseases), and studying membrane protein dynamics. Challenges in production include ensuring proper anchor attachment, stability, and functional conformation. Recent advances in glycoengineering and synthetic biology have improved the yield and fidelity of recombinant GPI proteins, enhancing their utility in therapeutic and diagnostic platforms. Their ability to integrate into cell membranes or liposomes also supports innovative delivery systems and artificial cell models.
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