| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPX2 |
| Uniprot No | P18283 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190aa |
| 氨基酸序列 | MAFIAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLUGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVAI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPX2重组蛋白的3篇参考文献示例：
---
1. **文献名称**: "Recombinant human GPX2 protein expression in E. coli and its antioxidant role in colorectal cancer cells"
**作者**: Müller S, et al.
**摘要**: 该研究报道了利用大肠杆菌系统高效表达重组人GPX2蛋白，并验证其在结肠癌细胞中通过调控ROS水平抑制氧化应激损伤的分子机制。
2. **文献名称**: "Structural and functional characterization of selenium-containing glutathione peroxidase 2 (GPX2) using recombinant protein technology"
**作者**: Zhang Y, et al.
**摘要**: 通过昆虫细胞表达系统获得重组GPX2蛋白，结合X射线晶体学解析其三维结构，揭示了硒代半胱氨酸活性中心对酶催化功能的关键作用。
3. **文献名称**: "GPX2 overexpression promotes tumor progression through ROS-NF-κB signaling: Insights from purified recombinant protein-based assays"
**作者**: Li H, et al.
**摘要**: 研究利用哺乳动物细胞表达纯化的GPX2重组蛋白，证明其通过ROS/NF-κB通路增强肿瘤细胞侵袭性，为GPX2的促癌机制提供了实验依据。
---
注：以上文献信息为示例性质，实际引用时需核对具体文献来源及准确性。建议通过PubMed或Web of Science以“GPX2 recombinant”为关键词检索最新研究。
**Background of GPX2 Recombinant Protein**
Glutathione peroxidase 2 (GPX2), a member of the glutathione peroxidase family, is a selenium-dependent antioxidant enzyme that plays a critical role in mitigating oxidative stress by catalyzing the reduction of hydrogen peroxide and lipid hydroperoxides. Primarily expressed in the gastrointestinal tract, liver, and epithelial tissues, GPX2 works in tandem with other GPX isoforms to protect cells from reactive oxygen species (ROS)-induced damage. Its function is closely linked to cellular homeostasis, inflammation regulation, and carcinogenesis, with studies implicating GPX2 dysregulation in colorectal cancer and other gastrointestinal disorders.
Recombinant GPX2 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, followed by purification to ensure high specificity and activity. This engineered protein retains the enzymatic properties of native GPX2. enabling researchers to study its biological roles in controlled settings. Applications include investigating oxidative stress mechanisms, exploring GPX2's dual role as both an antioxidant and a potential oncogene, and developing therapeutic strategies for diseases linked to oxidative damage.
The development of recombinant GPX2 has also advanced diagnostic and drug discovery efforts, particularly in cancers where GPX2 overexpression is observed. Its study provides insights into selenium metabolism, redox signaling pathways, and the balance between cytoprotection and pathological processes. As a research tool, recombinant GPX2 remains vital for deciphering its tissue-specific functions and translational potential in medicine.
×
