| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MGF; STAT5A |
| Entrez GeneID | 6776 |
| clone | 7F7A4 |
| WB Predicted band size | 90.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human Stat5 (AA: KAVDG[pTyr]VKPQIK). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Stat5抗体的3篇代表性文献及其摘要概括：
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1. **文献名称**：*STAT5 activation is critical for the initiation and maintenance of MYC-driven leukemia*
**作者**：Moriggl, R., et al.
**摘要**：该研究利用Stat5特异性抗体(针对磷酸化Tyr694位点)通过Western blot和流式细胞术，验证了Stat5的持续激活在MYC驱动的白血病发生中的关键作用，并揭示了其与下游靶基因的调控关系。
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2. **文献名称**：*Differential roles of STAT5 isoforms in breast cancer progression*
**作者**：Lin, J.X., et al.
**摘要**：研究通过Stat5a和Stat5b特异性抗体进行免疫沉淀和染色质免疫共沉淀(ChIP)，发现Stat5a促进乳腺癌细胞存活，而Stat5b抑制转移，揭示了亚型功能的差异性。
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3. **文献名称**：*Antibody-specific validation for STAT5B detection in human solid tumors*
**作者**：Hutchinson, J.N., et al.
**摘要**：本文系统比较了多种商业Stat5B抗体的特异性，通过敲除细胞系和免疫组化实验，筛选出高特异性抗体，为实体瘤中Stat5B蛋白的精准检测提供方法学支持。
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**注**：以上文献信息为模拟示例，实际引用需通过数据库(如PubMed)核对原文。若需具体文献，可补充实验背景或应用场景进一步筛选。
**Background of Stat5 Antibodies**
Stat5 (Signal Transducer and Activator of Transcription 5) is a transcription factor activated by cytokines, growth factors, and hormones. It exists in two isoforms, Stat5a and Stat5b, encoded by distinct genes but sharing ~96% amino acid sequence homology. Upon ligand-receptor binding, Stat5 is phosphorylated by JAK kinases, leading to dimerization, nuclear translocation, and regulation of target genes involved in cell proliferation, survival, differentiation, and immune responses.
Stat5 antibodies are essential tools for detecting and studying Stat5 proteins in research. They are designed to recognize specific epitopes, distinguishing between Stat5a and Stat5b, or detecting phosphorylated (activated) forms (e.g., p-Stat5 Tyr694/699). These antibodies are widely used in techniques like Western blotting, immunohistochemistry, flow cytometry, and chromatin immunoprecipitation (ChIP).
Dysregulation of Stat5 signaling is linked to cancers (e.g., leukemia, breast cancer), autoimmune disorders, and immune deficiencies. Stat5 antibodies help investigate its role in disease mechanisms, therapy resistance, and as a potential biomarker. For example, hyperactivation of Stat5 is observed in hematologic malignancies, making it a therapeutic target.
Commercial Stat5 antibodies vary in specificity, host species, and clonality. Validation across applications and species is critical, as cross-reactivity or batch variability may affect results. Researchers rely on these antibodies to explore Stat5’s dual roles in normal physiology and pathology, advancing insights into targeted therapies and cellular signaling networks.
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