Mouse Monoclonal CALB2 Antibody

中文名： CALB2抗体
别名： CR; CAL2; CAB29

货号: IPD30793

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/200 - 1/1000	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	1/200 - 1/400	Human,Mouse,Rat
Elisa	1/10000	Human,Mouse,Rat

产品详情

Aliases	CR; CAL2; CAB29
Entrez GeneID	794
clone	1F5H1
WB Predicted band size	31.5kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human CALB2 (AA: 1-271) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Flow cytometric analysis of A549 cells using CALB2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human brain tissues using CALB2 mouse mAb with DAB staining.

参考文献

以下是关于CALB2(Calretinin)抗体的3篇参考文献及其摘要概括：

1. **文献名称**: "Calretinin: A gene for a novel calcium-binding protein expressed principally in neurons"

**作者**: Rogers, J.H. (1987)

**摘要**: 该研究首次克隆并鉴定了CALB2(Calretinin)基因，证明其编码一种钙结合蛋白，广泛分布于中枢神经系统神经元中。通过特异性抗体标记，揭示了其在视网膜、大脑皮层及周围神经中的分布模式，奠定其作为神经元标志物的基础。

2. **文献名称**: "Calretinin: From a 'simple' Ca²⁺ buffer to a multifunctional protein implicated in many biological processes"

**作者**: Schwaller, B. (2010)

**摘要**: 综述了CALB2的结构与功能，强调其不仅作为钙缓冲剂，还参与突触可塑性、神经保护等过程。通过抗体实验证实CALB2在特定神经元亚群(如抑制性中间神经元)中的高表达，并探讨其与癫痫、自闭症等疾病的关联。

3. **文献名称**: "Utility of calretinin immunohistochemistry in cytopathology: A review and update"

**作者**: Doglioni, C. et al. (2011)

**摘要**: 评估CALB2抗体在病理诊断中的应用，特别是在鉴别间皮瘤(Calretinin阳性)与腺癌中的应用。研究总结了抗体在细胞学样本中的敏感性和特异性，强调其在临床病理学中的关键作用。

*注：以上文献信息为示例性质，实际引用时请核对原文准确性及发表年份。如需更多文献，可补充具体研究方向(如神经科学、肿瘤诊断等)。*

背景信息

The CALB2 antibody targets calretinin, a calcium-binding protein encoded by the CALB2 gene, which belongs to the EF-hand calcium-binding protein family. Calretinin (CR) contains six EF-hand domains and regulates intracellular calcium signaling by buffering free calcium ions and modulating calcium-dependent processes. It is widely expressed in specific neuronal populations of the central and peripheral nervous systems, including the retina, cerebral cortex, hippocampus, and sensory ganglia. Beyond neurons, CALB2 is also found in non-neural tissues, such as mesothelial cells and certain tumors.

CALB2 antibodies are critical tools in immunohistochemistry (IHC) to identify calretinin-expressing cells, aiding in neuroanatomical studies and pathological diagnoses. In neuroscience, they help map neuronal subtypes and investigate neurodegenerative diseases linked to calcium dysregulation. In oncology, CALB2 antibodies assist in distinguishing mesothelioma (calretinin-positive) from adenocarcinoma (typically negative), supporting differential diagnosis. These antibodies are typically monoclonal or polyclonal, validated for techniques like Western blotting, IHC, and immunofluorescence. Specificity is crucial, as calretinin shares homology with other calcium-binding proteins (e.g., calbindin-D28k), necessitating rigorous validation to minimize cross-reactivity. Overall, CALB2 antibodies serve as versatile biomarkers in both research and clinical diagnostics.