Mouse Monoclonal AIM2 Antibody

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

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     Immunofluorescence analysis of A549 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

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     Immunofluorescence analysis of MCF-7 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

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     Flow cytometric analysis of Hela cells using AIM2 mouse mAb (green) and negative control (red).    

The Absent in Melanoma 2 (AIM2) antibody targets AIM2. a cytosolic innate immune sensor critical for detecting double-stranded DNA (dsDNA) from pathogens or damaged host cells. AIM2 belongs to the PYHIN protein family and contains a N-terminal pyrin domain (PYD) and a C-terminal HIN200 domain, enabling dsDNA binding. Upon activation, AIM2 recruits ASC (apoptosis-associated speck-like protein) and procaspase-1 to form the AIM2 inflammasome, triggering caspase-1-mediated cleavage of pro-inflammatory cytokines IL-1β and IL-18. and inducing pyroptosis. This mechanism is vital for antimicrobial defense and inflammatory regulation.

AIM2 dysregulation is linked to autoimmune diseases (e.g., lupus, psoriasis), cancers, and infections. In cancer, AIM2 may act as a tumor suppressor by promoting apoptosis or exhibit oncogenic effects depending on context. Antibodies against AIM2 are essential tools for studying its expression, localization, and function in immune responses and disease pathogenesis. They enable techniques like Western blotting, immunofluorescence, and immunohistochemistry to explore AIM2's role in inflammasome activation, DNA damage responses, and therapeutic targeting. Research using AIM2 antibodies has advanced understanding of its dual roles in inflammation and cancer, highlighting its potential as a biomarker or therapeutic target.