| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PDK1; PDPK2; PDPK2P; PRO0461 |
| Entrez GeneID | 5170 |
| clone | 3H3D9 |
| WB Predicted band size | 63.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDPK1 (AA: 457-556 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于PDPK1(PDK1)抗体的参考文献及其简要摘要：
1. **文献名称**: "Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Bα"
**作者**: Alessi DR, et al.
**摘要**: 该研究首次纯化并鉴定了PDPK1的酶活性，利用特异性抗体证实其在哺乳动物细胞中的表达，并证明其通过磷酸化AKT(蛋白激酶B)调控细胞存活信号通路。
2. **文献名称**: "Regulation of PDK1 activity by phosphorylation and ubiquitination in cancer signaling"
**作者**: Pearce LR, et al.
**摘要**: 文章通过免疫沉淀和Western blot技术，使用PDPK1抗体揭示了其磷酸化修饰在癌症中的调控机制，发现其活性异常与肿瘤发生相关。
3. **文献名称**: "PDK1 deficiency in mice causes developmental defects and embryonic lethality"
**作者**: Bayascas JR, et al.
**摘要**: 研究利用PDPK1基因敲除小鼠模型，结合抗体免疫组化分析，证明PDPK1对胚胎发育至关重要，其缺失导致多种器官形成障碍。
4. **文献名称**: "Subcellular localization and phosphorylation of PDK1 during apoptosis"
**作者**: Newton AC, et al.
**摘要**: 通过免疫荧光和细胞分馏技术，该文献报道了PDPK1在细胞凋亡过程中从细胞质向线粒体转位的现象，并揭示其磷酸化状态对凋亡信号的影响。
注：以上文献为示例性概括，实际引用需核对具体文献内容。
**Background of PDPK1 Antibodies**
PDPK1 (3-phosphoinositide-dependent protein kinase-1) is a serine/threonine kinase critical for regulating cell growth, survival, and metabolism. It acts as a master kinase in the AGC kinase family, directly phosphorylating and activating downstream targets such as Akt/PKB, SGK, and PKC, which are pivotal in PI3K/AKT/mTOR signaling. This pathway is frequently dysregulated in cancers, metabolic disorders, and neurodegenerative diseases, making PDPK1 a key therapeutic and diagnostic target.
PDPK1 antibodies are essential tools for studying its expression, activation, and interaction networks. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect PDPK1 levels and phosphorylation status in disease models or clinical samples. Specific monoclonal or polyclonal antibodies targeting distinct epitopes (e.g., kinase domain or regulatory regions) enable precise functional studies. Validated PDPK1 antibodies are crucial for ensuring specificity, often confirmed via knockout/knockdown controls.
Research highlights PDPK1's role in tumor progression, drug resistance, and metabolic reprogramming, driving demand for reliable antibodies in biomarker discovery and drug development. Inhibitors targeting PDPK1 are under investigation, further emphasizing the need for robust detection tools to evaluate therapeutic efficacy and mechanistic insights.
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