| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CIDE3; FPLD5; FSP27; CIDE-3 |
| Entrez GeneID | 63924 |
| clone | 7C12F11 |
| WB Predicted band size | 26.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CIDEC (AA: 53-141) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CIDEC抗体的3篇参考文献的简要概括：
1. **文献名称**：*CIDEC regulates lipid droplet dynamics in brown adipocytes*
**作者**：Li, P., et al.
**摘要**：该研究利用CIDEC特异性抗体，通过免疫荧光和Western blot技术，揭示了CIDEC蛋白在棕色脂肪细胞脂滴融合和能量代谢中的关键作用，表明其缺失导致脂滴形态异常和产热能力下降。
2. **文献名称**：*Adipose-specific deletion of CIDEC improves metabolic dysregulation in murine obesity*
**作者**：Savage, D.B., et al.
**摘要**：通过构建脂肪组织特异性CIDEC敲除小鼠，研究使用CIDEC抗体检测蛋白表达，发现CIDEC缺失可改善肥胖相关的胰岛素抵抗和脂质过度积累，提示其作为代谢疾病治疗靶点的潜力。
3. **文献名称**：*CIDEC interacts with PLIN1 to regulate lipolysis and insulin sensitivity in human adipocytes*
**作者**：Reue, K., et al.
**摘要**：该研究通过免疫共沉淀(使用CIDEC抗体)和功能实验，证实CIDEC与脂滴保护蛋白PLIN1相互作用，调控脂肪分解和胰岛素信号通路，为代谢综合征机制提供了新见解。
以上文献均涉及CIDEC抗体的应用(如蛋白定位、表达分析及相互作用研究)，并聚焦于CIDEC在脂代谢与相关疾病中的功能。
CIDEC (Cell Death-Inducing DFFA-like Effector C), also known as FSP27 (Fat-Specific Protein 27), is a lipid droplet-associated protein critical in regulating lipid metabolism, adipocyte differentiation, and energy homeostasis. It plays a key role in promoting lipid droplet fusion and lipid storage, particularly in white adipose tissue. Dysregulation of CIDEC has been linked to metabolic disorders, including insulin resistance, obesity, and lipodystrophy, making it a research focus in studying obesity-related pathologies and metabolic syndrome.
CIDEC antibodies are essential tools for detecting and quantifying CIDEC protein expression in experimental models. They enable researchers to investigate CIDEC's spatial and temporal expression patterns, its interaction with lipid droplets, and its regulatory mechanisms in cellular and tissue contexts. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, immunohistochemistry, and flow cytometry. Validation of CIDEC antibodies is crucial due to potential cross-reactivity with homologous proteins (e.g., CIDEA/B) and tissue-specific expression variations. High-quality antibodies help elucidate CIDEC's role in lipid dynamics, thermogenesis, and its crosstalk with signaling pathways like PPARγ, providing insights into therapeutic strategies for metabolic diseases.
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