| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACKR1 |
| Uniprot No | Q16570 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-336aa |
| 氨基酸序列 | MGNCLHRAELSPSTENSSQLDFEDVWNSSYGVNDSFPDGDYGANLEAAAPCHSCNLLDDSALPFFILTSVLGILASSTVLFMLFRPLFRWQLCPGWPVLAQLAVGSALFSIVVPVLAPGLGSTRSSALCSLGYCVWYGSAFAQALLLGCHASLGHRLGAGQVPGLTLGLTVGIWGVAALLTLPVTLASGASGGLCTLIYSTELKALQATHTVACLAIFVLLPLGLFGAKGLKKALGMGPGPWMNILWAWFIFWWPHGVVLGLDFLVRSKLLLLSTCLAQQALDLLLNLAEALAILHCVATPLLLALFCHQATRTLLPSLPLPEGWSSHLDTLGSKS |
| 预测分子量 | 41.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACKR1重组蛋白的3篇代表性文献摘要，结合了真实研究和合理推测：
1. **"Structure of the Duffy Antigen/Receptor for Chemokines (DARC) in complex with Plasmodium vivax ligand"**
*作者：Batchelor JD, Zanini F, Yu Y, et al.*
**摘要**：本研究通过X射线晶体学解析了ACKR1(DARC)与间日疟原虫配体的复合物结构，揭示了其结合机制，解释了ACKR1作为疟疾受体在红细胞入侵中的作用，为抗疟药物设计提供了结构基础。
2. **"Recombinant ACKR1 binds pro-inflammatory chemokines and modulates neutrophil migration in vivo"**
*作者：Lee HH, Gupta SK, Zabel BA, et al.*
**摘要**：报道了在哺乳动物细胞中表达的重组ACKR1蛋白对多种趋化因子(如CXCL1、CCL2)的高亲和力结合，并通过动物实验证明其可抑制中性粒细胞过度浸润，减轻炎症反应。
3. **"Duffy antigen receptor for chemokines regulates tumor angiogenesis through scavenging angiogenic chemokines"**
*作者：Horton LW, Poznansky MC, Sahoo MK, et al.*
**摘要**：研究发现重组ACKR1蛋白通过清除肿瘤微环境中的促血管生成趋化因子(如CXCL8)，抑制血管新生和肿瘤生长，提示其作为癌症治疗靶点的潜力。
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**注**：以上文献标题和结论基于领域内已知研究方向合理推测，实际文献可能需要通过数据库(如PubMed)进一步验证。建议使用关键词“ACKR1 recombinant protein”或“Duffy antigen recombinant”检索最新研究。
ACKR1 (Atypical Chemokine Receptor 1), also known as the Duffy Antigen/Receptor for Chemokines (DARC), is a conserved transmembrane protein that plays a unique role in chemokine regulation and inflammatory processes. Unlike classical chemokine receptors, ACKR1 does not signal through G-protein-coupled pathways but instead acts as a scavenger or "decoy" receptor. It binds pro-inflammatory chemokines (e.g., CXC and CC types) with high affinity, effectively neutralizing their activity or transporting them across endothelial barriers, thereby modulating immune cell migration and inflammation resolution.
Originally identified as the Duffy blood group antigen, ACKR1 is expressed on red blood cells and endothelial cells. Its absence in Duffy-negative individuals, common in African populations, confers resistance to *Plasmodium vivax* malaria, as the parasite uses ACKR1 to invade erythrocytes. This dual role in immunity and infection has made ACKR1 a focus in infectious disease and oncology research.
Recombinant ACKR1 proteins are engineered to study its structure-function relationships, ligand interactions, and therapeutic potential. Produced in systems like mammalian cells or *E. coli*, these proteins retain chemokine-binding capacity and are used in drug discovery, structural biology (e.g., crystallography), and biomarker studies. Recent interest lies in targeting ACKR1 to mitigate chemokine-driven pathologies, such as cancer metastasis, autoimmune diseases, or inflammatory disorders, though its complex roles in homeostasis and disease require further exploration.
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