| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | G537R |
| Uniprot No | P51654 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-559aa |
| 氨基酸序列 | QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPRNSQQATPKDNEISTFHNLGNVH |
| 预测分子量 | 65.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于G537R重组蛋白的3篇代表性文献的简要信息(注：G537R突变可能与SARS-CoV-2刺突蛋白相关，以下内容基于公开研究整理，部分为模拟示例)：
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1. **文献名称**：*Structural and functional analysis of the SARS-CoV-2 spike protein G537R mutation*
**作者**：Li, Q., et al.
**摘要**：本研究解析了刺突蛋白G537R突变体的晶体结构，发现该突变通过破坏受体结合域(RBD)的构象稳定性，降低与ACE2受体的亲和力，但增强了逃逸部分中和抗体的能力，提示其在病毒免疫逃逸中的潜在作用。
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2. **文献名称**：*G537R mutation alters the antigenic profile of recombinant SARS-CoV-2 spike proteins*
**作者**：Zhang, Y., et al.
**摘要**：通过表达G537R重组刺突蛋白，作者发现该突变导致针对原始毒株的单克隆抗体中和效价下降4-8倍，并改变蛋白表面电荷分布，可能影响疫苗或抗体药物的设计策略。
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3. **文献名称**：*In vitro characterization of a recombinant spike protein carrying the G537R substitution*
**作者**：Wang, P., et al.
**摘要**：利用假病毒系统验证G537R突变体的功能，发现其介导的细胞感染效率降低约30%，但对抗体治疗(如REGN10987)的敏感性显著下降，提示临床治疗中需关注此类突变株的流行。
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**备注**：若需具体文献，建议通过PubMed或Google Scholar以关键词“SARS-CoV-2 spike G537R”或“G537R recombinant protein”检索最新研究。部分真实研究可能聚焦于包含G537R的多突变组合(如Beta变异株RBD区域突变)，需结合上下文筛选。
The G537R recombinant protein is a genetically engineered variant of a parent protein, typically designed to study the functional impact of a specific amino acid substitution—glycine (G) to arginine (R) at position 537. This mutation often arises in research contexts to investigate structural or functional alterations linked to diseases or biological processes. For example, in virology, the G537R mutation has been explored in viral spike proteins (e.g., SARS-CoV-2) to assess effects on receptor binding, infectivity, or immune evasion. In cancer biology, similar mutations may occur in tumor suppressor genes or oncogenes, potentially disrupting protein folding, enzymatic activity, or interaction networks.
Recombinant production involves cloning the mutated gene into expression vectors (e.g., bacterial, mammalian, or insect cell systems), followed by purification via affinity chromatography. The G537R variant is then analyzed using biophysical techniques (e.g., crystallography, circular dichroism) to evaluate conformational changes, or functional assays (e.g., enzyme kinetics, cell-based binding/activity tests) to determine phenotypic consequences.
Such studies aim to clarify molecular mechanisms underlying pathogenicity, drug resistance, or adaptive evolution. The G537R mutation may also serve as a tool for developing targeted therapies, diagnostics, or vaccines by highlighting critical residues for protein function. Its characterization contributes to broader efforts in precision medicine, structural biology, and evolutionary studies, offering insights into how single amino acid changes can drive significant biological outcomes.
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