Mouse Monoclonal RAD18 Antibody

+ +

•  

   
     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

•  

   
     Flow cytometric analysis of K562 cells using RAD18 mouse mAb (green) and negative control (red).    

The RAD18 antibody is a research tool used to study the RAD18 protein, a key player in DNA damage response and repair mechanisms. RAD18. an E3 ubiquitin ligase, functions primarily in the replication stress response by coordinating post-replication repair (PRR) pathways. It forms a complex with RAD6 (UBE2A/UBE2B) to monoubiquitinate proliferating cell nuclear antigen (PCNA) at stalled replication forks, facilitating translesion DNA synthesis (TLS) to bypass DNA lesions. This process is critical for maintaining genome stability and preventing replication fork collapse. RAD18 also interacts with other repair proteins, such as BRCA1 and FANCD2. highlighting its role in crosslink repair and Fanconi anemia pathways.

Antibodies targeting RAD18 enable researchers to investigate its expression levels, subcellular localization, and interactions via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. Dysregulation of RAD18 has been implicated in cancer progression, chemoresistance, and genomic instability, making it a focus in oncology and DNA repair studies. Commercial RAD18 antibodies are typically validated for specificity across human, mouse, and rat models, with applications in both basic research and preclinical contexts. Understanding RAD18's regulatory mechanisms through antibody-based assays contributes to insights into carcinogenesis and potential therapeutic targeting of DNA repair pathways.