| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2B |
| Uniprot No | Q00403 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-316aa |
| 氨基酸序列 | MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL |
| 预测分子量 | 50.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GTF2B(TFIIB)重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *Purification and characterization of the human general transcription factor TFIIB*
**作者**: Ha, I., Roberts, S., Maldonado, E., Sun, X., Kim, L.U., & Green, M. R.
**摘要**: 该研究首次报道了人源TFIIB重组蛋白的克隆、表达及功能验证，证实其作为RNA聚合酶II转录起始的关键因子，能够介导启动子识别并增强转录复合体稳定性。
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2. **文献名称**: *Structure of the human TFIIB-related factor BRF1 reveals a new regulator of eukaryotic transcription*
**作者**: Kostrewa, D., Zeller, M.E., & Cramer, P.
**摘要**: 通过X射线晶体学解析了人源TFIIB及其相关重组蛋白BRF1的三维结构，揭示了其在RNA聚合酶III转录中的调控机制，强调了锌指结构域对DNA结合的关键作用。
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3. **文献名称**: *Human general transcription factor TFIIB: Conformational variability and interaction with VP16 activation domain*
**作者**: Nikolov, D.B., Chen, H., Halay, E.D., & Burley, S.K.
**摘要**: 该研究利用重组TFIIB蛋白分析了其与转录激活因子VP16的相互作用，提出TFIIB的构象变化可能作为转录调控的分子开关，影响转录起始复合体的组装效率。
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4. **文献名称**: *Recombinant TFIIB from yeast enhances in vitro transcription by RNA polymerase II*
**作者**: Pinto, I., Wu, W.H., & Na, J.G.
**摘要**: 通过重组技术表达了酵母TFIIB蛋白，并证明其能显著提升体外RNA聚合酶II的转录活性，揭示了其在真核生物基础转录机器中的保守功能。
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以上文献涵盖了GTF2B(TFIIB)重组蛋白的结构解析、功能机制及转录调控研究，可作为相关领域的基础参考。
**Background of GTF2B Recombinant Protein**
GTF2B (General Transcription Factor II B), also known as TFIIB, is a critical component of the RNA polymerase II (Pol II) transcription machinery in eukaryotes. It plays a central role in the initiation phase of mRNA synthesis by bridging promoter-bound TFIID (TATA-binding protein complex) and RNA Pol II, facilitating the assembly of the pre-initiation complex (PIC). Structurally, GTF2B contains a zinc ribbon domain and a core domain, which mediate interactions with DNA, TFIID, and Pol II, ensuring accurate transcription start site selection.
Recombinant GTF2B protein is engineered using heterologous expression systems (e.g., *E. coli* or insect cells) to produce a purified, functional form of the factor for *in vitro* studies. This protein retains the ability to bind promoter regions, recruit Pol II, and stabilize the PIC, making it indispensable for dissecting transcriptional mechanisms. Its recombinant form is widely used in biochemical assays (e.g., electrophoretic mobility shift assays), structural studies (cryo-EM/X-ray crystallography), and *in vitro* transcription reconstitution experiments.
Research involving GTF2B recombinant protein has advanced understanding of transcriptional dysregulation in diseases, including cancers and developmental disorders. Additionally, it serves as a tool for screening small molecules targeting transcriptional anomalies. By enabling precise analysis of TFIIB’s interactions and functions, recombinant GTF2B remains pivotal in both basic research and therapeutic development.
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