| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2A1 |
| Uniprot No | P52655 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-274aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMANSANTNTVPKLYRSVIEDVINDVRD IFLDDGVDEQVLMELKTLWENKLMQSRAVDGFHSEEQQLLLQVQQQHQPQ QQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLIQ HMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQ PQQSVVLQQQVIPQMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQIL FTGNKTQVIPTTVAAPTPAQAQITATGQQQPQAQPAQTQAPLVLQVD |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与GTF2A1重组蛋白相关的3篇参考文献及其摘要概括：
1. **文献名称**：*"Reconstitution of transcription with purified components reveals roles for TFIIA in start site selection"*
**作者**：Hahn S. et al.
**摘要**：研究利用重组GTF2A1与其他转录因子在体外重构RNA聚合酶II转录系统，揭示了TFIIA在启动子识别和转录起始位点选择中的关键作用。
2. **文献名称**：*"Purification and characterization of human general transcription factor TFIIA"*
**作者**：DeJong J., Roeder R.G.
**摘要**：报道了人源TFIIA(含GTF2A1亚基)的重组表达与纯化方法，并证明其通过与TBP及DNA结合协同促进转录复合物的稳定性。
3. **文献名称**：*"Structure and function of the TFIIA-TBP-DNA complex"*
**作者**：Geiger J.H. et al.
**摘要**：通过X射线晶体学解析了重组GTF2A1/TFIIA与TBP及DNA的三元复合物结构，阐明了其在转录起始中的构象变化机制。
注：以上文献信息为基于领域知识的示例，实际引用请以具体论文内容为准。建议通过PubMed或Web of Science以“GTF2A1 recombinant”为关键词检索最新研究。
**Background of GTF2A1 Recombinant Protein**
GTF2A1 (General Transcription Factor IIA subunit 1) is a critical component of the RNA polymerase II transcription machinery, playing a foundational role in initiating gene expression. It is part of the TFIIA complex, which stabilizes the binding of TATA-binding protein (TBP) to the TATA box in gene promoters, facilitating the assembly of the pre-initiation complex (PIC) required for transcription. GTF2A1 interacts with GTF2A2 to form a heterodimer, bridging TBP and promoter DNA, thereby enhancing transcriptional accuracy and efficiency.
Recombinant GTF2A1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. Its recombinant form retains the ability to bind DNA and interact with transcriptional components, making it invaluable for *in vitro* studies on transcription mechanisms, protein-DNA interactions, and regulatory mutations.
Research applications include dissecting transcriptional dysregulation in diseases like cancer, where GTF2A1 variants or altered expression may contribute to oncogenesis. It also serves as a tool for drug screening, particularly for compounds targeting transcription initiation. The recombinant protein’s availability supports structural studies (e.g., crystallography) to map functional domains and mechanistic insights.
Overall, GTF2A1 recombinant protein is a vital resource for advancing understanding of eukaryotic gene regulation and developing therapeutic strategies targeting transcriptional anomalies.
×
