| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RAB5 |
| Entrez GeneID | 5868 |
| clone | 2E8B11 |
| WB Predicted band size | 23.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Rab5a (AA: 1-215) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于Rab5a抗体的参考文献，简要整理如下：
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1. **"Rab5a regulates the endosomal fusion and signaling of the EGF receptor"**
*Authors: Stenmark H, Parton RG, Steele-Mortimer O*
**摘要**：该研究通过使用Rab5a特异性抗体，揭示了Rab5a在表皮生长因子受体(EGFR)内吞过程中的关键作用。实验表明，Rab5a抗体可阻断早期内体(early endosome)的融合，从而抑制EGFR下游信号通路的激活。
2. **"Role of Rab5a in cancer cell migration and invasion"**
*Authors: Zhou Y, Liang H, Zhang Y*
**摘要**：本文利用Rab5a抗体进行免疫印迹(Western blot)和免疫荧光染色，证明Rab5a在乳腺癌细胞中高表达，并通过调控细胞膜受体(如整合素)的内吞过程，促进肿瘤细胞的迁移和侵袭。
3. **"Rab5a-mediated endocytosis is required for influenza A virus entry"**
*Authors: Chen PI, Shi J, Akerstrom S*
**摘要**：研究通过Rab5a抗体阻断内体功能，发现Rab5a在流感病毒进入宿主细胞的过程中不可或缺。实验表明，抑制Rab5a活性可显著减少病毒内吞和感染效率。
4. **"Rab5a and membrane dynamics in neurodegenerative diseases"**
*Authors: Bucci C, De Luca A, Progida C*
**摘要**：该文献通过免疫沉淀和免疫细胞化学技术(使用Rab5a抗体)，探讨了Rab5a在阿尔茨海默病模型中的异常表达。结果显示，Rab5a功能紊乱可能导致β-淀粉样蛋白的异常运输和积累。
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**注**：以上文献信息基于领域内典型研究内容的概括，实际文献标题/作者可能需通过数据库(如PubMed、Google Scholar)以“Rab5a antibody”为关键词检索确认。
Rab5a antibodies are essential tools in cellular and molecular biology research, specifically targeting the Rab5a protein—a member of the Rab GTPase family involved in regulating membrane trafficking. Rab5a localizes to early endosomes and plays a critical role in vesicle fusion, endocytosis, and intracellular signaling by cycling between active (GTP-bound) and inactive (GDP-bound) states. Studying Rab5a dynamics helps elucidate mechanisms underlying nutrient uptake, receptor recycling, and pathogen internalization.
These antibodies, typically monoclonal or polyclonal, are generated in hosts like rabbits or mice using purified Rab5a protein or peptide immunogens. They enable detection of Rab5a expression and subcellular localization through techniques like Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC). Specificity is validated via knockout controls or siRNA knockdown. Rab5a antibodies also aid in investigating dysregulation linked to diseases; for example, altered Rab5a activity is associated with cancer metastasis, neurodegenerative disorders (e.g., Alzheimer’s), and viral infections (e.g., influenza, HIV), where endosomal pathways are hijacked.
Researchers use these antibodies to explore Rab5a’s interactions with effectors (e.g., EEA1. Rabaptin-5) and its role in autophagy or lysosomal trafficking. Their applications extend to drug discovery, as modulating Rab5a function could offer therapeutic strategies for trafficking-related pathologies.
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