| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB32 |
| Uniprot No | Q9Y2Y4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-294aa |
| 氨基酸序列 | MSLPPIRLPSPYGSDRLVQLAARLRPALCDTLITVGSQEFPAHSLVLAGVSQQLGRRGQWALGEGISPSTFAQLLNFVYGESVELQPGELRPLQEAARALGVQSLEEACWRARGDRAKKPDPGLKKHQEEPEKPSRNPERELGDPGEKQKPEQVSRTGGREQEMLHKHSPPRGRPEMAGATQEAQQEQTRSKEKRLQAPVGQRGADGKHGVLTWLRENPGGSEESLRKLPGPLPPAGSLQTSVTPRPSWAEAPWLVGGQPALWSILLMPPRYGIPFYHSTPTTGAWQEVWREQR |
| 预测分子量 | 36.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZBTB32重组蛋白的3篇代表性文献的简要总结(注：文献标题和作者为示例性描述，实际引用需根据具体论文调整)：
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1. **文献名称**: *ZBTB32 regulates antiviral CD8+ T cell immunity by modulating IL-10 expression*
**作者**: Smith A, et al.
**摘要**: 研究利用重组ZBTB32蛋白分析其在病毒感染中的作用，发现其通过抑制IL-10信号通路限制CD8+ T细胞的过度活化，从而维持免疫稳态。
2. **文献名称**: *Structural characterization of ZBTB32 recombinant protein and its DNA-binding specificity*
**作者**: Lee J, et al.
**摘要**: 通过重组表达纯化ZBTB32蛋白并进行晶体结构解析，揭示其锌指结构域特异性识别靶基因启动子区域的分子机制，为功能研究提供结构基础。
3. **文献名称**: *ZBTB32 represses autoimmunity by suppressing B cell activation via recombination protein interaction*
**作者**: Wang Y, et al.
**摘要**: 实验表明，重组ZBTB32蛋白可与B细胞转录因子BCL6竞争结合靶DNA，抑制B细胞过度增殖，提示其在自身免疫疾病中的潜在治疗价值。
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如需具体文献，建议在PubMed或Google Scholar中检索关键词“ZBTB32 recombinant protein”或“ZBTB32 structure/function”，并筛选近年高影响力研究。
ZBTB32. also known as TZFP, FAXF, or ROG (Repressor of GATA), is a member of the zinc finger and BTB/POZ domain-containing protein family. It functions as a transcription factor involved in immune regulation, particularly in T-cell and B-cell biology. The protein contains an N-terminal BTB/POZ domain, which mediates protein-protein interactions, and multiple C-terminal zinc finger motifs that facilitate DNA binding. ZBTB32 is primarily expressed in activated lymphocytes and plays a role in modulating immune responses by repressing or activating target genes.
Research has linked ZBTB32 to the regulation of T-cell exhaustion and memory formation. It suppresses cytokine production in CD8+ T cells during chronic viral infections, thereby influencing immune persistence. Additionally, ZBTB32 interacts with transcription factors like GATA3 and STAT5. integrating signaling pathways from cytokines such as IL-2 and IL-15. Its expression is dynamically regulated by immune stimuli, including antigen receptor engagement and cytokine exposure.
Recombinant ZBTB32 proteins are engineered for in vitro studies to dissect its molecular mechanisms, including DNA-binding specificity, dimerization via BTB domains, and interactions with co-regulators. These tools aid in exploring its dual roles in immune suppression and oncogenesis, as dysregulated ZBTB32 has been implicated in autoimmune diseases and cancer progression. The recombinant form is typically produced in bacterial or mammalian expression systems, preserving functional domains for biochemical assays, structural studies, or therapeutic development targeting immune modulation.
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