| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YTHDF2 |
| Uniprot No | Q9Y5A9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-579aa |
| 氨基酸序列 | SASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARPNNAYTAMSDSYLPSYYSPSIGFSYSLGEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPDAMFGQPGALGSTPFLGQHGFNFFPSGIDFSAWGNNSSQGQSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSAVGSGSITSNIVASNSLPPATIAPPKPASWADIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIGTWDNKGPVAKAPSQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQPTRWVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQEEEESVKKERQGRGK |
| 预测分子量 | 68.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YTHDF2重组蛋白的3篇代表性文献摘要：
1. **文献名称**：*N6-methyladenosine modulates messenger RNA translation efficiency*
**作者**：Wang X. et al.
**摘要**：该研究首次揭示YTHDF2重组蛋白通过识别mRNA上的m6A修饰，介导靶标mRNA的降解，从而调控基因表达。实验表明，YTHDF2通过招募CCR4-NOT复合体加速mRNA的脱腺苷酸化。
2. **文献名称**：*Structural basis for the discriminative recognition of N6-methyladenosine RNA by the YTH domain*
**作者**：Shi H. et al.
**摘要**：通过晶体结构分析，本研究解析了YTHDF2重组蛋白的YTH结构域与含m6A修饰RNA的结合模式，揭示了其特异性识别m6A的分子机制，并验证了关键氨基酸残基在结合中的作用。
3. **文献名称**：*YTHDF2 links m6A modification to tumor progression by regulating oncogenic transcripts*
**作者**：Zhu T. et al.
**摘要**：研究利用重组YTHDF2蛋白发现其在癌症中通过降解促癌基因mRNA(如MYC、EGFR)抑制肿瘤生长，证明YTHDF2的m6A依赖功能在癌症治疗中的潜在应用价值。
4. **文献名称**：*The role of YTHDF2 in the regulation of hematopoietic stem cells*
**作者**：Huang H. et al.
**摘要**：该研究发现YTHDF2重组蛋白通过调控造血干细胞中m6A修饰的mRNA稳定性，影响干细胞自我更新与分化，为血液系统疾病的机制研究提供了新方向。
以上文献涵盖YTHDF2的结构解析、分子机制及疾病关联，建议结合具体研究方向进一步筛选。
YTHDF2 (YTH Domain-Containing Family Protein 2) is a reader protein that specifically recognizes N6-methyladenosine (m6A), the most abundant internal chemical modification in eukaryotic mRNA. Discovered as part of the m6A epitranscriptomic regulatory system, YTHDF2 plays a critical role in post-transcriptional gene regulation by binding m6A-modified transcripts and directing them to cellular RNA degradation pathways, such as deadenylation and decay through recruitment of CCR4-NOT complexes. This function links YTHDF2 to diverse biological processes, including stem cell differentiation, immune response, and cancer progression.
Recombinant YTHDF2 protein is engineered for in vitro studies to dissect its molecular mechanisms. Typically produced in bacterial (e.g., E. coli) or mammalian expression systems, the recombinant protein retains the conserved YTH domain responsible for m6A binding and C-terminal regions mediating downstream effector interactions. Purification methods often involve affinity tags (e.g., His-tag) followed by chromatographic techniques to ensure high purity and activity.
Research applications include RNA-protein interaction assays (e.g., RIP-seq, electrophoretic mobility shift assays), structural studies (X-ray crystallography, cryo-EM), and drug screening for m6A-targeted therapies. Dysregulation of YTHDF2 is implicated in diseases like leukemia, glioblastoma, and metabolic disorders, making its recombinant form valuable for developing diagnostic tools or inhibitors. Recent studies also explore its role in viral infection cycles, as m6A modifications regulate viral RNA stability. The development of YTHDF2 recombinant proteins has accelerated mechanistic insights into dynamic m6A-mediated gene regulation and its therapeutic potential.
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