| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | EEN; CNSA1; SH3P8; SH3D2B |
| Entrez GeneID | 6455 |
| clone | 4B4C2 |
| WB Predicted band size | 41.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human SH3GL1 (AA: 12-119) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于SH3GL1抗体的3篇代表性文献及其摘要：
1. **文献名称**：*SH3GL1. a new player in endosomal dynamics and cancer pathogenesis*
**作者**：Smith A, et al.
**摘要**：该研究揭示了SH3GL1蛋白通过调控内体膜重塑参与肿瘤发生。利用特异性抗体证实SH3GL1在乳腺癌细胞中表达下调，其缺失导致EGFR信号通路异常激活，促进细胞迁移。
2. **文献名称**：*The SH3 domain protein SH3GL1 controls synaptic vesicle endocytosis*
**作者**：Chen L, et al.
**摘要**：通过免疫荧光和Western blotting技术，研究发现SH3GL1抗体标记的蛋白在神经元突触小泡内吞过程中起关键作用，其缺失导致突触传递效率显著下降。
3. **文献名称**：*Interaction profiling of SH3GL1 with CrkL in leukemia cells*
**作者**：Tanaka R, et al.
**摘要**：利用SH3GL1抗体进行免疫共沉淀实验，发现其与CrkL蛋白在慢性粒细胞白血病中存在特异性结合，提示二者可能共同调控BCR-ABL致癌信号通路。
4. **文献名称**：*Structural basis of SH3GL1-mediated membrane deformation*
**作者**：Wang X, et al.
**摘要**：通过冷冻电镜和抗体标记技术解析SH3GL1蛋白寡聚化结构，阐明其通过BAR结构域诱导细胞膜弯曲的分子机制，为靶向内体运输的药物开发提供依据。
(注：以上文献信息为示例性内容，实际文献需通过PubMed/Google Scholar检索确认)
SH3GL1 (SH3 domain-containing GRB2-like protein 1), also known as EEN-B1 or endophilin-A2. is a member of the endophilin-A family involved in membrane dynamics and intracellular trafficking. It contains an N-terminal BAR domain, which facilitates membrane curvature sensing, and a C-terminal SH3 domain that mediates protein-protein interactions. SH3GL1 plays critical roles in clathrin-mediated endocytosis, synaptic vesicle recycling, and receptor signaling by recruiting partners like dynamin and synaptojanin to membrane sites. Its functions are linked to neuronal development, cellular communication, and cancer progression, with dysregulation observed in neurodegenerative disorders and malignancies.
SH3GL1 antibodies are essential tools for studying these processes. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect SH3GL1 expression levels, localization, and interactions in cells or tissues. Researchers employ these antibodies to investigate SH3GL1's role in endocytosis, apoptosis, and pathological conditions such as Alzheimer's disease, where aberrant endocytic pathways are implicated. Commercially available SH3GL1 antibodies are typically raised against specific epitopes (e.g., human SH3GL1 amino acid residues 1-100) and validated for cross-reactivity in model organisms like mice. Proper controls, including knockout cell lines, are recommended to ensure specificity due to high homology within the endophilin-A family. These antibodies contribute to elucidating SH3GL1's molecular mechanisms and therapeutic potential.
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