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Intra-assay Precision (Precision within an assay): CV%<8% | ||
Three samples of known concentration were tested twenty times on one plate to assess. | ||
Inter-assay Precision (Precision between assays): CV%<10% | ||
Three samples of known concentration were tested in twenty assays to assess. |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean(ng/ml) | 25.39 | 24.46 | 26.31 | 26.65 | 26.31 | 24.19 |
SD | 0.027 | 0.025 | 0.031 | 0.041 | 0.035 | 0.037 |
CV(%) | 5.7 | 5.46 | 6.33 | 8.26 | 7.13 | 8.01 |
ng/ml | OD1 | OD2 | Average | Corrected |
200 | 3.228 | 3.104 | 3.166 | 3.032 |
100 | 2.045 | 2.169 | 2.107 | 1.973 |
50 | 1.001 | 0.931 | 0.966 | 0.832 |
25 | 0.469 | 0.507 | 0.488 | 0.354 |
12.5 | 0.268 | 0.276 | 0.272 | 0.138 |
6.25 | 0.196 | 0.204 | 0.200 | 0.066 |
3.12 | 0.168 | 0.161 | 0.165 | 0.031 |
0 | 0.136 | 0.132 | 0.134 |
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